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High-performance liquid chromatography–high-resolution time-of-flight mass spectrometry in the identification and determination of penicillin and tetracycline group antibiotics in food

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Abstract

A method of the identification and determination of 27 antibiotics of penicillin (15) and tetracycline (12) groups in food using high-resolution time-of-flight mass spectrometry combined with high-performance liquid chromatography and a simple and fast sample preparation procedure have been developed. The analytical range for penicillins is 3 (50)–300 (1000) μg/kg and for tetracyclines, 0.3 (1.5)–100 (200) μg/kg. A scheme of the identification and determination of antibiotics using the standard addition method has been proposed. The relative standard deviation of the results of analysis does not exceed 10%. The time of sample screening is 30–40 min, the time of the determination of the detected antibiotics is 2–3 h.

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Authors and Affiliations

  1. Federal Center for Animals Health, Yur’evets, Vladimir, 600901, Russia

    V. G. Amelin

  2. Vladimir State University, ul. Gor’kogo 87, Vladimir, 600000, Russia

    V. G. Amelin & A. I. Korotkov

  3. Bryansk Interregional Veterinary Laboratory, ul. Shosseinaya 7, Suponevo, Bryansk oblast, 241520, Russia

    A. I. Korotkov

Authors
  1. V. G. Amelin
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  2. A. I. Korotkov
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Correspondence to V. G. Amelin.

Additional information

Original Russian Text © V.G. Amelin, A.I. Korotkov, 2015, published in Zhurnal Analiticheskoi Khimii, 2015, Vol. 70, No. 11, pp. 1201–1207.

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Amelin, V.G., Korotkov, A.I. High-performance liquid chromatography–high-resolution time-of-flight mass spectrometry in the identification and determination of penicillin and tetracycline group antibiotics in food. J Anal Chem 70, 1383–1389 (2015). https://doi.org/10.1134/S1061934815110027

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  • DOI: https://doi.org/10.1134/S1061934815110027

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