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New Data on Taxonomic and Geographic Distribution of the trnLUAA Intron Deletion of Chloroplast DNA in Hedysarum L. (Fabaceae L.)

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Abstract

A significant length polymorphism (309–735 bp) and nucleotide variability were detected by the study of the intergenic spacer trnL-trnF of chloroplast DNA in Hedysarum species belonging to sections Hedysarum and Multicaulia (family Fabaceae). Among nineteen Hedysarum species analyzed, the region trnL-trnF was not investigated before for thirteen species. The trnL intron deletion was first discovered for four Hedysarum species: H. austrosibiricum, H. cisbaiсalense, H. theinum, and H. consanguineum. The chloroplast cytotype without deletion of the trnL intron was reported for the first time for H. alpinum. This paper proposes a hypothesis about the polyphyletic origin of the genus and mechanisms of the formation of the phenomena for the first time established for genus Hedysarum of heteroplasmy and chloroplast cytotype variability found in H. consanguineum and H. alpinum on the basis of the data of trnL intron labeling.

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ACKNOWLEDGMENTS

This work was carried out within the framework of the state task of the Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences Assessment of the Morphogenetic Potential of Plant Populations of North Asia by Experimental Methods, project no. AAAA-A17-117012610051-5.

We are grateful for the help in collecting the plant material to S.V. Asbaganov, I.A. Artemov, L.V. Buglova, E.V. Kobozeva, D.E. Nikonova, I.E. Smelyanskii, and I.V. Khan and to herbarium curators (NSK) V.M. Doron’kin and I.M. Deyun for kindly providing the herbarium samples for DNA analysis and L.Z. Lukmanova for assistance in the preparation of herbarium samples. To carry out the research, materials from the bioresource scientific collection of the Central Siberian Botanical Garden Herbarium of Higher Vascular Plants, Lichens, and Fungi (NSK) were used, UNU no. USU 440537.

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  1. Central Siberian Botanical Garden, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    N. S. Nuzhdina & O. V. Dorogina

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    A. A. Bondar

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  1. N. S. Nuzhdina
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Nuzhdina, N.S., Bondar, A.A. & Dorogina, O.V. New Data on Taxonomic and Geographic Distribution of the trnLUAA Intron Deletion of Chloroplast DNA in Hedysarum L. (Fabaceae L.). Russ J Genet 54, 1282–1292 (2018). https://doi.org/10.1134/S1022795418110108

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