Skip to main content
SpringerLink
Log in

Direct Shoot Organogenesis from Seedling Derived Shoot Tip Explants of Endangered Medicinal Plant Saussurea costus (Falc.) Lipsch.

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

An efficient direct in vitro plant regeneration protocol for Saussurea costus (kuth) was developed using shoot tip explants derived from in vitro grown seedlings to generate genetically uniform plants. Murashige and Skoog (MS) medium supplemented with 1.14 µM thiadiazuron and 2.68 µM naphthalene acetic acid was found to be most optimal for highest for average shoot regeneration (73.33%) with maximum average number of shoots (11.4) and average shoot length (4.17 cm). For in vitro multiplication, shoots cultured on MS medium supplemented with 4.44 µM benzyl adenine, 2.32 µM kinetin and 1.44 µM gibberellic acid were found to grow best with 20.7 average shoot multiplication rate after 5–6 weeks. MS medium supplemented with 2.46 µM indole butyric acid was found to be the best medium for rooting of microshoots (77.78% rooting with 6.0 average number of roots and 3.07 cm average root length). The in vitro raised plantlets were successfully acclimatized under ex vitro conditions. The true to type nature of the in vitro raised plants was confirmed using DNA based ISSR markers for assessment of genetic stability of micropropagated plants. The standardized micropropagation protocol under present study may be tuned towards mass production of roots which are the most economical part of the plant. This would be useful as prerequisite for carrying out any genetic transformation studies while concentrating on any desirable trait in the same herb.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Shah R (2006) Nature medicinal plants of Uttaranchal: herbs, grasses & ferns. Gyanodaya Prakashan, Nanital Uttarakhand

    Google Scholar 

  2. Kala CP, Dhyani PP, Sajwan BK (2006) Developing the medicinal plants sector in northern India: challenges and opportunities. J Ethnobiol Ethnomed 2:32–47

    Article  PubMed Central  Google Scholar 

  3. Anonymous (2000) Report of the task force on conservation and sustainable use of medicinal plants. Planning Commission, Government of India, New Delhi

    Google Scholar 

  4. Anonymous (2011) A review of the status of Saussurea costus. In: Convention on international trade in endangered species of wild fauna and flora, nineteenth meeting of the plants committee held at Geneva (Switzerland), pp 1–19

  5. Butola JS, Samant SS (2010) Saussurea species in Indian Himalayan region: diversity, distribution and indigenous uses. Int J Plant Biol 1(1):43–51

    Article  Google Scholar 

  6. Kapoor LD (2001) Handbook of ayurvedic medicinal plants. CRC Press, Washington, DC

    Google Scholar 

  7. Nautiyal S, Maikuri RK, Rao KS, Saxena KG (2003) Ethnobotany of the tolcha bhotia tribe of the buffer zone villages in Nanda Devi bioshpere reserve India. J Econ Taxon Bot 27:119–142

    Google Scholar 

  8. Robinson A, Kumar TV, Sreedhar E, Naidu VG, Krishna SR, Babu KS, Srinivas PV, Rao JM (2008) A new sesquiterpene lactone from the roots of Saussurea lappa: structure-anticancer activity study. Bioorg Med Chem Lett 18(14):4015–4017

    Article  CAS  PubMed  Google Scholar 

  9. Mohiuddin GG, Nawchoo IA, Wafai A (2005) Seed germination studies on Saussurea costus (Falc.) Lipsch–a threatened medicinal plant of N.W. Himalaya. Indian J Plant Physiol 10(4):394–396

    Google Scholar 

  10. Lal N, Ahuja PS (1988) Clonal propagation of Picrorhiza kurroa Royle ex Benth. by shoot tip culture. Plant Cell Rep 7:201–205

    Article  Google Scholar 

  11. Giri A, Paramir SA, Kumar APV (1993) Somatic embryogenesis and plant regeneration from callus cultures of Aconitum heterophyllum Wall. Plant Cell, Tissue Organ Cult 32:313–318

    Article  Google Scholar 

  12. Joshi M, Dhar U (2003) In vitro propagation of Saussurea obvallata (DC.) Edgew. an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Rep 21:933–939

    Article  CAS  PubMed  Google Scholar 

  13. Soni M, Kaur R (2014) Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa. Physiol Mol Biol Plants 12:81–85

    Google Scholar 

  14. Arora R, Bhojwani SS (1989) In vitro propagation and low temperature storage of Saussurea lappa C.B. Clarke–an endangered medicinal plant. Plant Cell Rep 8:44–47

    Article  CAS  PubMed  Google Scholar 

  15. Gailite A, Klavina D, Ievinsh G (2010) In vitro propagation of an endangered plant Saussurea esthonica. Environ Exp Biol 8:43–48

    Google Scholar 

  16. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissues. Focus 12:13–15

    Google Scholar 

  17. Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. Wiley, New York

    Google Scholar 

  18. Subotic A, Jevremovic S, Grubisic D (2009) Influence of cytokinin on in vitro morphogenesis in root cultures of Centaurium erythraea-valuable medicinal plant. Sci Hortic 120:386–390

    Article  CAS  Google Scholar 

  19. Johnson TS, Badari NS, Narayana DBA (1997) Rapid in vitro propagation of Saussurea lappa: an endangered medicinal. In Vitro Cell Dev Biol Plant 33:128–130

    Article  Google Scholar 

  20. Bhandari S, Dobhal U, Bisht S, Bisht NS (2013) In vitro conservation of Saussurea costus: an endangered medicinal plant. Vegetos 26(1):67–71

    Google Scholar 

  21. Hosseini R, Parsa M (2007) Micropropagation of Aloe vera L. grown in South Iran. Pak J Biol Sci 10(7):1134–1137

    Article  CAS  PubMed  Google Scholar 

  22. Balaraju K, Agastian P, Ignacimuthu S (2009) Micropropagation of Swertia chirata Buch. Hams. ex Wall: a critically endangered medicinal herb. Acta Physiol Plant 31(3):487–494

    Article  Google Scholar 

  23. Bhandari AK, Negi JS, Bisht VK, Bharti MK (2010) In vitro propagation of Aloe vera: plant with medicinal properties. Nat Sci 8(8):174–176

    Google Scholar 

  24. Pant M, Bisht P, Gusain MP (2010) De novo shoot organogenesis from cultured root explants of Swertia chirata Buch.-Hamex Wall.: an endangered medicinal plant. Nat Sci 8(9):244–252

    Google Scholar 

  25. Zolman BK, Yoder A, Bartel B (2000) Genetic analysis of indole-3-butyric acid responses in Arabidopsis thaliana reveals four mutant classes. Genetics 156:1323–1337

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Srivastva SK, Srivastva N (2004) In vitro multiplication of Paedaria foetida. J Plant Biochem Biotechnol 13(1):89–91

    Article  Google Scholar 

  27. Gou B, Gao M, Lui CZ (2007) In vitro propagation of an endangered medicinal plant Saussuria involucrate Kar. et Kir. Plant Cell Rep 26(3):261–265

    Article  CAS  Google Scholar 

  28. Martin KP, Pachathundikandi SK, Zhang CI, Slater A, Madassery J (2006) RAPD analysis of a variant of banana (Musa sp.) cv. Grande naine and its propagation via shoot tip culture. Vitro Cell Dev Biol Plant 42(2):188–192

    Article  CAS  Google Scholar 

  29. Ilczuk A, Jacygrad E (2016) In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers. Vitro Cell Dev Biol-Plant 52(4):379–390

    Article  CAS  Google Scholar 

  30. Yuan XF, Dai ZH, Wang XD, Zhao B (2009) Assessment of genetic stability in tissue cultured products and seedlings of Saussurea involucrate by RAPD and ISSR markers. Biotech Lett 31:1279–1287

    Article  CAS  Google Scholar 

  31. Purohit S, Rawat V, Jugran AK, Singh RV, Bhatt ID, Nandi SK (2015) Micropropagation and genetic fidelity analysis in Valeriana jatamansi clones. J Appl Res Med Aromat Plants 2:15–20

    Google Scholar 

Download references

Acknowledgement

The authors are grateful to the National Medicinal Plant Board, New Delhi, India for providing financial assistance by sanctioning research project vide sanction order No. Z.18017/187/CSS/R&D/HP-01/2011-12-NMPB.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan, HP, 173 230, India

    Sneh Sharma, Rajnish Sharma, Parul Sharma & Kalpna Thakur

  2. Department of Forest Products, Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan, HP, 173 230, India

    Bhupender Dutt

Authors
  1. Sneh Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rajnish Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Parul Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kalpna Thakur
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bhupender Dutt
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rajnish Sharma.

Ethics declarations

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

Additional information

Significance Statement

This is a first report on efficient direct regeneration protocol of S. costus using shoot tip explants. Being roots as an economical part, obtained results will be helping in mass production of roots and for carrying genetic transformation studies.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sharma, S., Sharma, R., Sharma, P. et al. Direct Shoot Organogenesis from Seedling Derived Shoot Tip Explants of Endangered Medicinal Plant Saussurea costus (Falc.) Lipsch.. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 755–764 (2019). https://doi.org/10.1007/s40011-018-0983-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-018-0983-z

Keywords

Search

Navigation