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Determination of heavy metal ions by an amperometric biosensor based on glucose oxidase immobilized onto single-walled carbon nanotubes/Nile blue nanocomposite

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Abstract

An enzymatic electrochemical biosensor was prepared based on glucose oxidase (GOD) immobilized on single-walled carbon nanotubes/Nile blue (Nb–SWCNTs) nanocomposite cross-linked with glutaraldehyde on graphite electrode. The function of glucose biosensor is based on the electrocatalytic reduction of H2O2 generated by the reaction of the enzyme with glucose molecules. The biosensor was characterized by Fourier transform infrared (FTIR) spectroscopy, emission scanning (FESEM), UV–Vis spectrometry, and voltammetric and amperometric methods. Parameters influencing the performance of the biosensor, namely pH and applied potential, were optimized. Inhibition was carried out for the detection of Hg2+ and Pb2+ species under optimized condition. Inhibition investigations showed that the type of inhibition for both the abov- mentioned heavy metal ions were reversible and competitive.

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Authors and Affiliations

  1. Department of Environmental Process Engineering, Sharif University of Technology, Tehran, Iran

    Mirali Jahangirzadeh Varjovi & S. Mehdi Borghei

  2. Department of Analytical Chemistry, Faculty of Chemistry, Urmia University, Urmia, Iran

    Reza Emamali Sabzi

  3. Institute of Biotechnology, Urmia University, Urmia, Iran

    Reza Emamali Sabzi

Authors
  1. Mirali Jahangirzadeh Varjovi
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  2. Reza Emamali Sabzi
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  3. S. Mehdi Borghei
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Correspondence to Reza Emamali Sabzi.

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Varjovi, M.J., Sabzi, R.E. & Borghei, S.M. Determination of heavy metal ions by an amperometric biosensor based on glucose oxidase immobilized onto single-walled carbon nanotubes/Nile blue nanocomposite. J IRAN CHEM SOC 15, 1765–1774 (2018). https://doi.org/10.1007/s13738-018-1374-3

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  • DOI: https://doi.org/10.1007/s13738-018-1374-3

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