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Antagonistic Activity of Nocardia brasiliensis PTCC 1422 Against Isolated Enterobacteriaceae from Urinary Tract Infections

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Abstract

The main drawback of current antibiotic therapies is the emergence and rapid increase in antibiotic resistance. Nocardiae are aerobic, Gram-positive, catalase-positive, non-motile actinomycetes. Nocardia brasiliensis was reported as antibiotic producer. The purpose of the study was to determine antibacterial activity of N. brasiliensis PTCC 1422 against isolated Enterobacteriaceae from urinary tract infections (UTIs). The common bacteria from UTIs were isolated from hospital samples. Antimicrobial susceptibility test was performed for the isolated pathogens using Kirby–Bauer disk diffusion method according to clinical and Laboratory Standards Institute guideline. Antagonistic activity of N. brasiliensis PTCC 1422 was examined with well diffusion methods. Supernatant of N. brasiliensis PTCC 1422 by submerged culture was analyzed with gas chromatography–mass spectrometry. Isolated strains included Escherichia coli, Klebsiella pneumoniae, Serratia marcescens and Proteus mirabilis. The most common pathogen isolated was E. coli (72.5 %). Bacterial isolates revealed the presence of high levels of antimicrobial resistances to ceftriaxone and low levels of resistance to cephalexin. Supernatant of N. brasiliensis PTCC 1422 showed antibacterial activity against all of the isolated microorganisms in well diffusion method. The antibiotic resistance among the uropathogens is an evolving process, so a routine surveillance to monitor the etiologic agents of UTI and the resistance pattern should be carried out timely to choose the most effective empirical treatment by the physicians. Our present investigation indicates that the substances present in the N. brasiliensis PTCC 1422 could be used to inhibit the growth of human pathogen. Antibacterial resistance among bacterial uropathogen is an evolving process. Therefore, in the field on the need of re-evaluation of empirical treatment of UTIs, our present. The study has demonstrated that N. brasiliensis PTCC 1422 has a high potential for the treatment of UTIs.

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Acknowledgments

This study was supported by Department of Microbiology, Faculty of Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran

    Hossnieh Kafshdar Jalali, Abdolreza Salamatzadeh, Arezou Kafshdar Jalali & Khosro Issazadeh

  2. Department of Biology, Faculty of Science, Sari Branch, Farhangian University, Sari, Iran

    Abdolreza Salamatzadeh

  3. Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran

    Hamed Haddad Kashani

  4. Department of Molecular and Cellular Biology, Faculty of Science, University of Mazandaran, Babolsar, Iran

    Salman Ahmadi Asbchin

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  1. Hossnieh Kafshdar Jalali
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  2. Abdolreza Salamatzadeh
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  3. Arezou Kafshdar Jalali
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Correspondence to Abdolreza Salamatzadeh.

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Hossnieh Kafshdar Jalali, Abdolreza Salamatzadeh, Arezou Kafshdar Jalali, Hamed Haddad Kashani, Salman Ahmadi Asbchin and Khosro Issazadeh declare that they have no conflict of interest.

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Jalali, H.K., Salamatzadeh, A., Jalali, A.K. et al. Antagonistic Activity of Nocardia brasiliensis PTCC 1422 Against Isolated Enterobacteriaceae from Urinary Tract Infections. Probiotics & Antimicro. Prot. 8, 41–45 (2016). https://doi.org/10.1007/s12602-016-9207-0

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