Abstract
The present study was carried out to determine the effect of probiotic, Bacillus subtilis, on ash and calcium contents of tibia bone in unchallenged and challenged broiler chicks with Salmonella enteritidis. In a completely randomized design, 160 chicks were divided into four groups. Each group had four replicates with 10 birds each. Treatments were control group, probiotic-treated group, challenged group and challenged probiotic-treated group. Ash and calcium contents of tibia at 21 and 42 days of age were determined. At 21 days of age, the highest contents of ash and calcium were related to probiotic-treated group and the lowest means to challenged chicks (P < 0.05). At this period, inclusion of probiotic to diet of challenged chick increased (P < 0.05) ash and calcium contents of tibia. With increases in age, the negative effects of challenging and beneficial effects of probiotic on bone mineralization diminished; since at 42 days of age, challenging or probiotic treatment had no effect on ash and calcium contents of tibia.
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Acknowledgments
The author is grateful to the Islamic Azad University, Science and Research Branch for research funding support and to Agricultural, Medical and Industrial Research School, NSTR (AEOI) for supplying the basal diet and allowing use of poultry unit and to Baltec Company (presentation of Biochem Company, Germany) for providing probiotic Gallipro sample. The author also thanks all staffs (Mr. Zargaran and Mrs. Shakorzadeh) in the poultry unit for the assistance in the care and feeding of the chicks used in this research.
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Sadeghi, A.A. Bone Mineralization of Broiler Chicks Challenged with Salmonella enteritidis Fed Diet Containing Probiotic (Bacillus subtilis). Probiotics & Antimicro. Prot. 6, 136–140 (2014). https://doi.org/10.1007/s12602-014-9170-6
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DOI: https://doi.org/10.1007/s12602-014-9170-6