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Vitamin C and E in Fluoride Induced Uterine Oxidative Stress and Apoptotic Gene Expression in Wistar Rats

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Abstract

Management of fluoride (F) driven abnormalities is a great challenge. The present study emphasized on the abrogation of F facilitated uterine dysfunction by vitamin C and E. In the present investigation Wistar rats were fed with 200 ppm NaF at the dose of 10 mg/kg body weight orally for 28 days along with co-treatment with vitamin C and E at the doses of 50 mg/kg body weight and 15 mg/kg body weight, respectively. F treated group presented a concomitant decrease in the activity of uterine superoxide dismutase (SOD) and catalase (CAT). F ingestion developed up-regulation of tumor necrosis factor (TNF-α) and nuclear factor κB (NF-κB). The pro-apoptotic genes Bax and p53 expressions in uterine tissue were raised in response to fluoridation followed by suppression of anti-apoptotic gene Bcl-2. Vitamin C and E employed its constructive action against F intoxication by weakening TNF-α and NF-κB followed by a promotion of Bcl-2 expression along with Bax and p53 suppression. However, present investigation established the anti-oxidative, anti-inflammatory and anti-apoptotic events of Vitamin C and E.

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Authors and Affiliations

  1. Department of Biomedical Laboratory Science and Management, Vidyasagar University, Midnapore, West Bengal, 721102, India

    Pikash Pratim Maity, Moumita Dash, Arindam Dey, Moulima Maity & Sandip Chattopadhyay

  2. Clinical Nutrition and Dietetics Division, Vidyasagar University, Midnapore, West Bengal, 721102, India

    Pikash Pratim Maity, Moumita Dash, Arindam Dey, Moulima Maity & Sandip Chattopadhyay

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  1. Pikash Pratim Maity
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Maity, P.P., Dash, M., Dey, A. et al. Vitamin C and E in Fluoride Induced Uterine Oxidative Stress and Apoptotic Gene Expression in Wistar Rats. Proc Zool Soc 74, 189–197 (2021). https://doi.org/10.1007/s12595-021-00362-w

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