Abstract
Sugarcane often suffers from low-temperature stress in Guangxi, China. In this study, the cold tolerance potential of 20 new sugarcane varieties was evaluated on the basis of chlorophyll content (Chl), chlorophyll fluorescence parameters, plasma membrane permeability (PMP) and malondialdehyde (MDA) content, and visual scoring. The study was conducted at the experimental field of Fushui Field Station of Guangxi University. Following the period of cold stress, the Chl of sugarcane varieties decreased, while PMP and MDA contents increased. Moreover, the chlorophyll fluorescence parameters (Fo, Fm, Fv/Fm, Fv/Fo) also showed changes as a result of cold stress. However, these fluctuations significantly varied among different varieties. Likewise, the cold tolerance index (CTI) of the sugarcane varieties ranged from 0.03 to 0.76 depicting a great variability in the cold stress tolerance of varieties under evaluation. The correlation analysis of physiological indicators with cold tolerance parameters revealed that CTI was positively correlated with PMP and MDA and negatively correlated with Chl and its fluorescence parameters. Based on overall evaluation, eight varieties, viz. GUC44, CP88-1762, CP96-1257, GUC50, GX13-4201, GX13-4222, CP97-2730, and CP05-1762, were identified as a highly cold tolerant, while seven varieties were categorized as mild tolerant, five as susceptible, two as mild highly tolerant, and two as mild susceptible. This study offers an insight into physiological response of sugarcane against cold stress and an all-inclusive approach to screen sugarcane genotypes against this widespread issue of concern. Moreover, it is expected that this study will provide some promising sugarcane germplasm resources with better cold stress tolerance.
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References
Agati, G., Z.G. Cerovic, and I. Moya. 2000. The effect of decreasing temperature up to chilling values on the in vivo F685/F735 chlorophyll fluorescence ratio in Phaseolus vulgaris and Pisum sativum: The role of the photosystem I contribution to the 735 nm fluorescence band. Photochemistry and Photobiology 72: 75–84.
Artus, N.N., M. Uemura, P.L. Steponkus, and S.J. Gilmour. 1996. Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance. Proceedings of the National Academy of Sciences 93 (23): 13404–13409.
Badeck, F.W., and F. Rizza. 2015. A combined field/laboratory method for assessment of frost tolerance with freezing tests and chlorophyll fluorescence. Agronomy 5 (1): 71–88.
Chen, A.K., R.H. Han, and D.Y. Li. 2010. A comparison of two methods for electrical conductivity about plant leaves. Journal of Guangdong Education Institute 30 (5): 88–91.
Chen, N. 1990. The identification of cool resistance by means of electical conductivity in sugarcane. Sugarcane and Canesugar 6: 14–20.
Chen, S.Y. 1991. Injury of membrane lipid peroxidation to plant cell. Plant Physiology Communications 27 (2): 84–90.
Dan, O., F.J.F. Fan, Z.X. Zhou, and J.S. Gao. 2017. Comparison and evaluation on cold-tolerance of ten varieties in Sect. Leuce. Journal of Northeast Forestry University 1: 4.
Guo, C.F. 2006. The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. Journal of Fujian Institute of Education 62 (1): 62–76.
He, Y., Z. Tan, and M. Ding. 2009. Infrequent disaster of the cold and freezing disaster and their impacts on sugarcane production in Guangxi. Journal of Catastrophology 24 (1): 68–72.
Krause, G.H., and E. Weis. 1991. Chlorophyll fluorescence and photosynthesis: the basics. Annual Review of Plant Biology 42 (1): 313–349.
Liu, X., B. Zhao, H. Shen, J. Xu, and X. Gao. 2017. Comprehensive evaluation of cold resistance of twenty rhododendron varieties under cold stress. Northern Horticulture 5: 60–66.
Lyons, J.M. 1973. Chilling injury in plants. Annual Review of Plant Physiology 24 (1): 445–466.
Ming, L.I., H.C. Tian, and Z.G. Huang. 2017. Research on the development status of sugarcane industry in China. Sugar Crops of China 1: 20.
Mishra, A., K.B. Mishra, and I.I. Höermiller. 2011. Chlorophyll fluorescence emission as a reporter on cold tolerance in Arabidopsis thaliana accessions. Plant Signaling & Behavior 6 (2): 301–310.
Peguero-Pina, J.J., F. Morales, and E. Gil-Pelegrín. 2008. Frost damage in Pinus sylvestris L. stems assessed by chlorophyll fluorescence in cortical bark chlorenchyma. Annals of Forest Science 65 (8): 1.
Rapacz, M., M. Tyrka, and W. Kaczmarek. 2010. Photosynthetic acclimation to cold as a potential physiological marker of winter barley freezing tolerance assessed under variable winter environment. Journal of Agronomy and Crop Science 194 (1): 61–71.
Rizza, F., D. Pagani, and A.M. Stanca. 2010. Use of chlorophyll fluorescence to evaluate the cold acclimation and freezing tolerance of winter and spring oats. Plant Breeding 120 (5): 389–396.
Rizza, F., D. Pagani, A.M. Stanca, and L. Cattivelli. 2001. Use of chlorophyll fluorescence to evaluate the cold acclimation and freezing tolerance of winter and spring oats. Plant Breeding 120 (5): 389–396.
Stewart, R.R., and J.D. Bewley. 1980. Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology 65 (2): 245–248.
Tan, Z.K., C.H. Huang, C.L. Meng, et al. 2014. Study on the grade indexes of sugarcane chilly injury or freezing injury and loss. Chinese Agricultural Science Bulletin 30 (28): 169–181.
Tang, S.Y., L.T. Yang, and Y.R. Li. 2012a. Response of different sugarcane genotypes to low temperature and evaluation for cold tolerance of sugarcane. Chinese Journal of Tropical Crops 33 (4): 635–641.
Tang, S.Y., L.T. Yang, and Y.R. Li. 2012b. Correlation between changes of photosynthetic characteristics of different sugarcane varieties and their cold tolerance under low temperature. Guihaia 32 (5): 679–685.
Thomas, H., and A.R. James. 1993. Freezing tolerance and solute changes in contrasting genotypes of loliumperenne L. acclimated to cold and drought. Annals of Botany 72 (3): 249–254.
Wang, G., and Z. Guo. 2005. Responses of photorespiration to chilling stress in rice with different chilling tolerance. Acta Agronomica Sinica 31 (5): 673–676.
Xiao, Y. 1984. Studies on the cold tolerance of maize at different growth stages. Acta Agronomica Sinica 10 (1): 41–50.
Yang, R.Z., Y.R. Li, W.Z. Wang, et al. 2011. Evaluation for cold tolerance of sugarcane under rain frost condition. Southwest China Journal of Agricultural Sciences 24 (3): 1065–1071.
Zhang, B., L. Yang, and Y. Li. 2011. Comparison of physiological and biochemical characteristics related to cold resistance in sugarcane under field conditions. Acta Agronomica Sinica 37 (3): 496–505.
Zhang, M., R. Chen, J. Lu, J. Luo, and J. Xu. 1999. Effects of low temperature stress on the chlorophyll a fluorescence induction kinetics in the seedling of sugarcane. Journal of Fujian Agricultural University 28 (1): 1–7.
Zhang, J., X. Wu, and R. Niu. 2012. Cold-resistance evaluation in 25 wild grape species. Vitis 51 (4): 153–160.
Zhou, Y.H., L.F. Huang, and J.Q. Yu. 2004. Effects of sustained chilling and low light on gas exchange, chlorophyll fluorescence quenching and absorbed light allocation in cucumber leaves. Acta Photophysiologica Sinica 30 (2): 153–160.
Zhu, P.J., X.H. Pang, Q.L. Tan, et al. 2019. Effects of chilling on photosynthesis and photosynthetic pigment contents in leaves of different sugarcane varieties. Chinese Journal of Tropical Crops 40 (5): 875–881.
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Xu, Y., Chen, Z., Huang, Z. et al. Field Evaluation of New Promising Sugarcane Cultivars for Cold Tolerance in Guangxi, China. Sugar Tech 22, 1007–1017 (2020). https://doi.org/10.1007/s12355-020-00853-8
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DOI: https://doi.org/10.1007/s12355-020-00853-8