Abstract
The tribe Rhynchosporeae comprises the genera Rhynchospora and Pleurostachys and has never been studied using molecular techniques. The objective of this study was to use an analysis of trnL-F sequences to evaluate the hypothesized taxonomic divisions within the Rhynchosporeae including the monophyly of the genera and the soundness of the subgenera and sections. A total of 44 ingroup species were studied, 41 of Rhynchospora representing 22 of Kükenthal’s 28 sections, and three of Pleurostachys. Five outgroup species from other genera were also included. The cladistic analysis of 50 trnL intron and trnL-F intergenic spacer sequences resulted in 16 trees and a strict consensus tree. The Rhynchosporeae form two well-supported primary clades with several well-supported smaller clades, many of which agree with previously hypothesized sections. Pleurostachys is embedded within the second primary clade.
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Literature Cited
Araujo, A. C. 2001. Revisão de Rhynchospora Vahl sect. Pluriflorae Kük. (Cyperaceae). Doctoral dissertation. University of São Paulo, Brazil.
Bentham, G. and J. D. Hooker. 1883. Genera Plantarum 3. Lovell Reeve & Co., London.
Bruhl, J. J. 1995. Sedge genera of the world: relationships and a new classification of the Cyperaceae. Austral. Syst. Bot. 8: 125–305.
Clarke, C. B. 1908. New Genera and Species of Cyperaceae. Kew Bull., Add. Ser. 8: 1–196.
Doyle, J. J., and J. L. Doyle. 1987. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.
Gale, S. 1944. Rhynchospora sect. Eurhynchospora in Canada, the United States and the West Indies. Rhodora 46: 80–134; 159–197; 207–249; 255–278
Goetghebeur, P. 1998. Cyperaceae. Pp. 141–190. In K. Kubitzki, H. Huber, P. J. Rudall, P. S. Stevens, and T. Stuetzel (eds.), The Families and Genera of Vascular Plants. Springer-Verlag, Berlin.
Goloboff, P. 1999. NONA (NO NAME) version 2.0. Published by the author, Tucumán, Argentina (http://www.cladistics.com/aboutNona.htm).
———. 1999. Analyzing large data sets in reasonable times: solutions for composite optima. Cladistics 154: 415–428.
Grisebach, A. H. R. 1864. Flora of the British West Indian Islands. Lovell Reeve & Co., London.
Guaglianone, E. R. 2001. Contribución al estudio del Género Rhynchospora (Cyperaceae) V. Sección Longirostres en América Austral. Darwiniana 39: 287–342.
Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41: 95–98.
Koyama, T. 1972. Cyperaceae—Rhynchosporeae and Cladieae. In: Botany of the Guayana Highland. Part IX. Mem. New York Bot. Gard. 23: 23–89.
Kükenthal, G. 1949. Vorarbeiten zu einer Monographie der Rhynchosporoideae. Rhynchospora. Bot. Jahrb. Syst. 74: 375–509.
———. Vorarbeiten zu einer Monographie der Rhynchosporoideae. Rhynchospora. Bot. Jahrb. Syst. 75: 90–126, 127–195.
———. Vorarbeiten zu einer Monographie der Rhynchosporoideae. Rhynchospora. Bot. Jahrb. Syst. 75: 273–314.
———. Vorarbeiten zu einer Monographie der Rhynchosporoideae. Pleurostachys. Bot Jahrb. Syst. 75: 451–497.
Kunth, C. S. 1837. Enumeratio Plantarum 2. Cyperographia Synoptica. J. G. Collae, Stuttgart and Tübingen.
Muasya, A. M., J. Bruhl, D. A. Simpson, A. Culham and M. W. Chase. 2000. Suprageneric phylogeny of Cyperaceae: A combined analysis. Pp. 593–601. In: K. Wilson and D. Morrison (eds.) Monocots: Systematics and Evolution. CSIRO: Melbourne.
———, D. A. Simpson, M. W. Chase and A. Culham. 1998. An assessment of suprageneric phylogeny in Cyperaceae using rbcL DNA sequences. Pl. Syst. Evol. 211: 257–271.
Nees von Esenbeck, C. G. D. 1835. Uebersicht der Cyperaceengattungen (Synopsis Generum Cyperacearum). Linnaea 9: 282.
———, ———. 1842. Cyperaceae. In: C. F. P. Martius, Flora brasiliensis. 2(1): 1–226.
Nixon, K. C. 2002. Winclada version 1.0.08. Published by the author, Ithaca, NY (http://www.cladistics.com/).
Pell, S. K. 2004. Molecular Systematics of the Cashew Family (Anacardiaceae). Doctoral Dissertation. Louisiana State University.
Quiagen. 2005. DNeasy Plant Handbook. Qiagen Inc., Valencia, CA. http://www.qiagen.com.
Struwe, L., M. Thiv, J. W. Kadereit, A. S.-R. Pepper, T. J. Motley, P. J. White, J. H. E. Rova, K. Potgieter, and V. A. Albert. 1998. Saccifolium (Saccifoliaceae), an endemic of Sierra de La Neblina on the Brazilian–Venezuelan frontier, is related to a temperate-alpine lineage of Gentianaceae. Harvard Papers in Botany 3: 199–214.
Taberlet, P., L. Gielly, G. Pautou, and J. Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17: 1105–1109.
Thomas, W. W. 1984. The systematics of Rhynchospora sect. Dichromena. Mem. New York Bot. Gard. 37: 1–116.
———. 1992. A synopsis of Rhynchospora (Cyperaceae) in Mesoamerica. Brittonia 44: 14–44.
———. 1994. Rhynchospora Vahl (Cyperaceae). In: G. Davidse, M. Souza S. and A. O. Chater (eds.), Flora Mesoamericana 6: 404–422. Universidad Nacional Autónoma de México, México, D. F.; Missouri Botanical Garden, St. Louis; The Natural History Museum, London.
———. 1998. Rhynchospora (Cyperaceae). Pp. 610–629. In: J. A. Steyermark, P. E. Berry, and B. K. Holst, (eds.) Flora of the Venezuelan Guayana, Volume 4, Caesalpiniaceae–Ericaceae. Missouri Botanical Garden Press, St. Louis.
——— and M. Alves. (2008) A Revision of the Genus Pleurostachys (Cyperaceae): Preliminary Results. In: R. Naczi (ed.) Proceedings of “Sedges 2002: International Conference on Uses, Diversity, and Systematics of Cyperaceae” Ann. Missouri Bot. Gard. (in press)
Acknowledgments
We thank P. Delprete for the collection of fresh material dried in silica gel. We thank the Lewis B. and Dorothy Cullman Program for Molecular Systematics Studies of The New York Botanical Garden for making it possible for us to carry out the research. We thank the William and Lynda Steere Herbarium of The New York Botanical Garden for permission to remove samples from specimens for DNA extraction and analysis. Particular thanks are extended to K. Cameron, S. Pell, I. Sanchez-del Pino, and D. Little for help and guidance in the laboratory.
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Thomas, W.W., Araújo, A.C. & Alves, M.V. A Preliminary Molecular Phylogeny of the Rhynchosporeae (Cyperaceae). Bot. Rev 75, 22–29 (2009). https://doi.org/10.1007/s12229-008-9023-7
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DOI: https://doi.org/10.1007/s12229-008-9023-7