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Isolation and characterization of cellulolytic bacteria from the Stain house Lake, Antarctica

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Abstract

The main aim was to evaluate the occurrence of cellulolytic bacteria from the Stain house Lake, located at Admiralty Bay, Antarctica. Thick cotton string served as a cellulose bait for the isolation of bacteria. A total of 52 bacterial isolates were recovered and tested for their cellulase activity, and two of them, isolates CMAA 1184 and CMAA 1185, showed significant cellulolytic activity on carboxymethylcellulose agar plates. Phylogenetic analysis placed the isolates into the Bacillus 16S ribosomal RNA gene subclade. Both isolates produced a cold-active cellulase which may play a crucial role in this extreme environment.

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References

  • Antony R, Krishnan KP, Laluraj CM, Thamban M, Dhakephalkar PK, Engineer AS, Shivaji S (2012) Diversity and physiology of culturable bacteria associate with a coastal Antarctic ice core. Microb Res 167(6):372–380

    Article  CAS  Google Scholar 

  • D’Amico S, Collins T, Marx JC, Feller G, Gerday C (2006) Psychrophilic microorganisms: challenges for life. Embo Rep 7(4):385–389

    Article  PubMed Central  PubMed  Google Scholar 

  • de Menezes AB, Lockhart RJ, Cox MJ, Allison HE, McCarthy AJ (2008) Cellulose degradation by micromonosporas recovered from freshwater lakes and classification of these actinomycetes by DNA gyrase B gene sequencing. Appl Environ Microb 74(22):7080–7084

    Article  Google Scholar 

  • Henshaw T, Laybourn-Parry J (2002) The annual patterns of photosynthesis in two large, freshwater, ultra-oligotrophic Antarctic lakes. Polar Biol 25(10):744–752

    Google Scholar 

  • Iyo AH, Forsberg CW (1999) A cold-active glucanase from the ruminal bacterium Fibrobacter succinogenes S85. Appl Environ Microb 65(3):995–998

    CAS  Google Scholar 

  • Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M, Na H, Park S-C, Jeon YS, Lee J-H, Yi H, Won S, Chun J (2011) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  PubMed  Google Scholar 

  • Kuznetsov SI, Dubinina GA, Lapteva NA (1979) Biology of oligotrophic bacteria. Annu Rev Microbiol 33:377–387

    Article  CAS  PubMed  Google Scholar 

  • Miller L, Berger T (1985) Bacterial identification by gas chromatography of whole cell fatty acids. Hewlett-Packard, Palo Alto

    Google Scholar 

  • Ormerod JG (1983) Bacteria in their natural environments. In: Slater JH, Whittenbury R, Wimpeny JWT (eds) Microbes in their natural environments. Cambridge University Press, Cambridge, pp 463–482

    Google Scholar 

  • Roberts MS, Nakamura LK, Cohan FM (1994) Bacillus mojavensis sp. nov., distinguishable from Bacillus subtilis by sexual isolation, divergence in DNA sequence, and differences in fatty acid composition. Int J Syst Bacteriol 44(2):256–264

    Article  CAS  PubMed  Google Scholar 

  • Ruegger MJS, Tauk-Tornisielo SM (2004) Atividade da celulase de fungos isolados do solo da Estação Ecológica de Juréia-Itatins, São Paulo, Brasil. Rev Bras Bot 2:205–211

    Article  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc, Newark

    Google Scholar 

  • Soares FL Jr, Melo IS, Dias ACF, Andreote FD (2012) Cellulolytic bacteria from soils in harsh environments. World J Microbiol Biotechnol 28(5):2195–2203

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zucchi TD, Almeida LG, Cônsoli FL (2011) Culturable bacterial diversity associated with cysts of Eurhizococcus brasiliensis (Hempel) (Hemiptera: Margarodidae). World J Microbiol Biotechnol 27(4):791–797

    Article  Google Scholar 

  • Zucchi TD, Kim B-Y, Bonda ANV, Goodfellow M (2012) Actinomadura xylanilytica sp. nov., a novel actinomycete isolate from soil. Int J Syst Evol Micr 63(2):576–580

    Google Scholar 

Download references

Acknowledgments

This study was made possible with the financial and logistic support of PROANTAR and Marine of Brazil. This is part of the IPY activity 403 “MIDIAPI Microbial Diversity of Terrestrial and Maritime Ecosystems in Antarctic Peninsula.” TDZ is also grateful to FAPESP for providing the funding for developing this research (grants 11/50243-1 and 11/14333-6).

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Authors and Affiliations

  1. Embrapa Environment, CP 69, 13820-000, Jaguariúna, São Paulo, Brazil

    Itamar S. Melo, Tiago D. Zucchi, Rafael E. Silva, Elke S. D. Vilela & Mirian Lobo Sáber

  2. Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Luiz H. Rosa

  3. Oceanographic Institute, University of São Paulo, São Paulo, São Paulo, Brazil

    Vivian H. Pellizari

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  1. Itamar S. Melo
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  2. Tiago D. Zucchi
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  3. Rafael E. Silva
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  4. Elke S. D. Vilela
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  5. Mirian Lobo Sáber
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  6. Luiz H. Rosa
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  7. Vivian H. Pellizari
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Corresponding author

Correspondence to Itamar S. Melo.

Electronic supplementary material

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Supplementary Table 1

Fatty acid composition (%) of the isolates (DOCX 12 kb)

Supplementary Figure 1

Cellulolytic activity of isolates CMAA 1184 and CMAA 1185 estimated using the dinitrosalicylic acid method [DNS; filter paper as substrate in 50 mM sodium acetate buffer (pH 5.0)]. Bars, standard error (DOCX 72 kb)

Supplementary Figure 2

Cellulolytic activity of isolates CMAA 1184 and CMAA 1185 estimated using the dinitrosalicylic acid method [DNS; filter paper as substrate in 50 mM sodium acetate buffer (pH 3-10)]. Bars, standard error (DOCX 67 kb)

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Melo, I.S., Zucchi, T.D., Silva, R.E. et al. Isolation and characterization of cellulolytic bacteria from the Stain house Lake, Antarctica. Folia Microbiol 59, 303–306 (2014). https://doi.org/10.1007/s12223-013-0295-x

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  • DOI: https://doi.org/10.1007/s12223-013-0295-x

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