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Culturable bacterial diversity associated with cysts of Eurhizococcus brasiliensis (Hempel) (Hemiptera: Margarodidae)

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Abstract

The ground-pearl Eurhizococcus brasiliensis is an important insect pest of grapes. Nowadays, its biology is still barely known and studies related to its secondary symbionts are virtually non-existent. Our main goal was to evaluate the bacterial diversity associated with cysts of E. brasiliensis using a culture-dependent approach. Six different isolation media were used and shown to be suitable for culturing bacteria associated with E. brasiliensis. A total of 39 bacteria strains were isolated and classified into 10 different morphotypes. The ISP-4 medium was the most suitable for bacteria isolation from cysts of the ground-pearl, providing the highest number of morphotypes. 16S rDNA gene analysis demonstrated a high diversity of bacteria associated with cysts, with six Pseudomonas chlororaphis isolates (the most predominant morphotype) and three different morphotypes of Bacillus spp. as the most representative genera. The phylogenetic analysis showed close affinity between the isolated morphotypes and bacterial strains usually isolated from plant and soil samples. Sphingopyxis and Stenotrophomonas were isolated for the first time from arthropods. Although it was not possible to determine the primary source of infection by these bacteria, our data suggests that these microorganisms may be transitory and acquired from the environment.

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Acknowledgments

Authors would like to thank Dr Marcos Botton (EMBRAPA) for providing samples of Eurhizococcus brasiliensis, Jason M. Dillon for English revision and an anonymous reviewer for his comments and suggestions. The authors are in debt to CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and FAPESP (Fundação de Amparo à Pesquisa de São Paulo) for providing fellowships to LGA (grant 119867/2009-5) and TDZ (grant 07/58712-5), respectively. FLC is also thankful to FAPESP for providing the necessary funds for developing this research (grant 07/59019-1).

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  1. Lab de Interações em Insetos, Depto de Entomologia and Acarologia, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo (ESALQ/USP), Av Pádua Dias 11, CP 9, 13418-900, Piracicaba, SP, Brazil

    Tiago D. Zucchi, Luis G. Almeida & Fernando L. Cônsoli

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  1. Tiago D. Zucchi
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  2. Luis G. Almeida
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  3. Fernando L. Cônsoli
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Correspondence to Tiago D. Zucchi or Fernando L. Cônsoli.

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Zucchi, T.D., Almeida, L.G. & Cônsoli, F.L. Culturable bacterial diversity associated with cysts of Eurhizococcus brasiliensis (Hempel) (Hemiptera: Margarodidae). World J Microbiol Biotechnol 27, 791–797 (2011). https://doi.org/10.1007/s11274-010-0518-0

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  • DOI: https://doi.org/10.1007/s11274-010-0518-0

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