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Development of a Highly Sensitive Competitive Indirect Enzyme-Linked Immunosorbent Assay for Detection of Acrylamide in Foods and Water

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Abstract

Acrylamide (AA) has been classified as a probable human carcinogen and forms in certain foods, particularly plant-based foods that are rich in carbohydrates and low in proteins, during processing or cooking at high temperatures. In this study, polyclonal antibodies were raised against a hapten derived from acrylamide and 3-mercaptobenzoic acid (3-MBA). An indirect competitive enzyme-linked immunosorbent assay was developed to rapidly quantify AA in complex food matrices and water. The assay was very specific to the AA-3-MBA derivative and showed no cross-reactivity to asparagine, the main precursor of AA formation in foods, aspartic acid, AA, or 3-MBA. The assay was very sensitive with a limit of detection of 5.0 ng/g in model for food matrices to 0.1 μg/L in water. Good recoveries for AA were observed in all matrices tested, and the results using this method were comparable to those obtained from mass spectrometry methods including Food Analysis Performance Assessment Scheme control samples and results for different food products.

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Acknowledgments

The authors give sincere thanks to Dr. Xu-Liang Cao and Dr. Zhongwen Wang for their helpful comments during the editing of this manuscript.

Conflict of Interest

Gurmit Singh has no conflict of interest. Beth Brady has no conflict of interest. Terry Koerner has no conflict of interest. Adam Becalski has no conflict of interest. Tony Zhao has no conflict of interest. Sherry Feng has no conflict of interest. Samuel Benrejeb Godefroy has no conflict of interest. Anne-Catherine Huet has no conflict of interest. Philippe Delahaut has no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.

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Authors and Affiliations

  1. Food Research Division, Bureau of Chemical Safety, Health Canada, AL2203D, Ottawa, ON, Canada, K1A 0K9

    Gurmit Singh, Beth Brady, Terry Koerner, Adam Becalski, Tony Zhao & Sherry Feng

  2. Food Directorate-HPFB, Health Canada, Ottawa, ON, Canada, K1A 0K9

    Samuel Benrejeb Godefroy

  3. Département Santé, Centre d’Economie Rurale, Rue du point du jour, 8, 6900, Marloie, Belgium

    Anne-Catherine Huet & Philippe Delahaut

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  1. Gurmit Singh
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  2. Beth Brady
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  3. Terry Koerner
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  4. Adam Becalski
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  5. Tony Zhao
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  6. Sherry Feng
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  7. Samuel Benrejeb Godefroy
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  8. Anne-Catherine Huet
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  9. Philippe Delahaut
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Corresponding author

Correspondence to Gurmit Singh.

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Singh, G., Brady, B., Koerner, T. et al. Development of a Highly Sensitive Competitive Indirect Enzyme-Linked Immunosorbent Assay for Detection of Acrylamide in Foods and Water. Food Anal. Methods 7, 1298–1304 (2014). https://doi.org/10.1007/s12161-013-9749-7

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  • DOI: https://doi.org/10.1007/s12161-013-9749-7

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