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MicroRNA expression in ACTH-producing pituitary tumors: up-regulation of microRNA-122 and -493 in pituitary carcinomas

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Abstract

MicroRNAs (miRNAs) are involved in cell proliferation, differentiation, and apoptosis, and can function as tumor suppressor genes or oncogenes. The expression of miRNAs in pituitary carcinomas has not been previously examined. We used miRNA profiling with 1,145 probes to study miRNA expression in normal anterior pituitary (6 cases), adrenocorticotropin (ACTH)-producing adenomas (8 cases), and ACTH-producing pituitary carcinomas (two cases). Real-time RT-PCR and in situ hybridization were used to confirm and independently validate miRNAs that were significantly up-regulated or down-regulated between the pituitary tissues. There were more miRNAs up- (188) or down-regulated (160) between adenomas and normal pituitaries compared to carcinomas and normal pituitaries (92 up- and 91 down-regulated) or between carcinomas and adenomas (46 up- and 52 down-regulated). Both real-time RT-PCR and in situ hybridization showed significant up-regulation of miRNA-122 between pituitary carcinomas and adenomas. MiRNA-493 was also up-regulated in carcinomas compared to ACTH adenomas. Analysis of genes that miRNA-493 interacts with included LGALS3 and RUNX2 (http://microrna.sanger.ac.uk) both of which have been shown to have roles in pituitary tumor cell growth. These results provide information about marker miRNAs that may lead to further insights into the regulation of pituitary tumor growth and development.

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Acknowledgment

This article was supported in part by the Jarislowsky Foundation and K68660 OTKA grant (Hungary). The authors thank the National Pituitary Agency for the anti-pituitary hormone antibodies.

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Authors and Affiliations

  1. Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN, 55905, USA

    Gail Stilling, Shuya Zhang, Long Jin, Alberto Righi, Bernd W. Scheithauer & Ricardo V. Lloyd

  2. Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, USA

    Zhifu Sun

  3. Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, Italy

    Alberto Righi

  4. 2nd Department of Internal Medicine, State Health Center, Budapest, Hungary

    Gάbor Kovācs

  5. Department of Endocrinology, Barts and the London Medical School, London, UK

    Mάrta Korbonits

  6. St. Michaels Hospital, Toronto, CA, USA

    Kalman Kovacs

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  1. Gail Stilling
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Correspondence to Ricardo V. Lloyd.

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Stilling, G., Sun, Z., Zhang, S. et al. MicroRNA expression in ACTH-producing pituitary tumors: up-regulation of microRNA-122 and -493 in pituitary carcinomas. Endocr 38, 67–75 (2010). https://doi.org/10.1007/s12020-010-9346-0

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  • DOI: https://doi.org/10.1007/s12020-010-9346-0

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