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Effects of Dietary Supplementation with Vitamin C and Vitamin E and Their Combination on Growth Performance, Some Biochemical Parameters, and Oxidative Stress Induced by Copper Toxicity in Broilers

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Abstract

This study investigated effects of dietary supplementation with vitamin C, vitamin E on performance, biochemical parameters, and oxidative stress induced by copper toxicity in broilers. A total of 240, 1-day-old, broilers were assigned to eight groups with three replicates of 10 chicks each. The groups were fed on the following diets: control (basal diet), vitamin C (250 mg/kg diet), vitamin E (250 mg/kg diet), vitamin C + vitamin E (250 mg/kg + 250 mg/kg diet), and copper (300 mg/kg diet) alone or in combination with the corresponding vitamins. At the 6th week, the body weights of broilers were decreased in copper, copper + vitamin E, and copper + vitamin C + vitamin E groups compared to control. The feed conversion ratio was poor in copper group. Plasma aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities, iron, copper concentrations, and erythrocyte malondialdehyde were increased; plasma vitamin A and C concentrations and erythrocyte superoxide dismutase were decreased in copper group compared to control. Glutathione peroxidase, vitamin C, and iron levels were increased; aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and copper levels were decreased in copper + vitamin C group, while superoxide dismutase, glutathione peroxidase, and vitamin E concentrations were increased; aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase were decreased in copper with vitamin E group compared to copper group. The vitamin C concentrations were increased; copper, uric acid, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and malondialdehyde were decreased in copper + vitamin C + vitamin E group compared to copper group. To conclude, copper caused oxidative stress in broilers. The combination of vitamin C and vitamin E addition might alleviate the harmful effects of copper as demonstrated by decreased lipid peroxidation and hepatic enzymes.

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Acknowledgment

Financial support for this Project (Project No. 2008/40) was provided by the Kirikkale University Research Fund.

Some parts of this study were presented as an abstract at the 6th National Veterinary Biochemistry Congress, Kars, Turkey.

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Veterinary Medicine, Kirikkale University, 71450, Yahsihan/Kirikkale, Turkey

    Miyase Cinar & Ozkan Duru

  2. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey

    Ebru Yildirim

  3. Department of Physiology, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey

    A.Arzu Yigit & Nurgul Atmaca

  4. Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Kirikkale University, Kirikkale, Turkey

    Ilkay Yalcinkaya

  5. Department of Medical Biochemistry, Faculty of Medicine, Kirikkale University, Kirikkale, Turkey

    Uçler Kisa

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Cinar, M., Yildirim, E., Yigit, A. et al. Effects of Dietary Supplementation with Vitamin C and Vitamin E and Their Combination on Growth Performance, Some Biochemical Parameters, and Oxidative Stress Induced by Copper Toxicity in Broilers. Biol Trace Elem Res 158, 186–196 (2014). https://doi.org/10.1007/s12011-014-9926-6

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