Abstract
This study sought to obtain internal wool lipid extracts rich in ceramides from different wool types. Extraction methods, i.e., Soxhlet extraction with different organic solvents and supercritical fluid extraction with CO2 using several polarity modifiers such as MeOH or EtOH, were optimized. The internal wool lipid content varied from 0.2 to 1.9% (based on wool weight) with a ceramide content ranging from 15 to 30% (based on extract weight). The Spanish and Russian Merino wool extracts were the richest in ceramide compounds. TLC-FID was used to quantify the different internal wool lipid extracts. A new experimental protocol that enabled us to identify most of the different ceramide types is presented. These internal wool lipid extracts, especially the ones with a high ceramide content, may be regarded as an alternative source of animal ceramides, which could be of value in the cosmetic and dermopharmaceutical industries.
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References
Coderch, L., C. Soriano, A. de la Maza, P. Erra, and J.L. Parra, Chromatographic Characterization of Internal Polar Lipids from Wool, J. Am. Oil Chem. Soc. 72:715–720 (1995).
Schaefer, H., and T.E. Redelmeier, Skin Barrier: Principles in Percutaneous Penetration, Karger, Basel, 1996, pp. 55–58.
Elias, P.M., Lipids and the Epidermal Permeability Barrier, Arch. Dermatol. Res. 270:95–117 (1981).
Yang, L., M. Mao-Qiang, M. Tljebini, P.M. Elias, and K.R. Feingold, Topical Stratum Corneum Lipids Accelerate Barrier Repair After Tape Stripping, Solvent Treatment and Some but Not All Types of Detergent Treatment, Br. J. Dermatol. 133:679–685 (1995).
Mao-Qiang, M., K.R. Feingold, C.R. Thornfeldt, and P.M. Elias, Optimization of Physiological Lipid Mixtures for Barrier Repair, J. Invest. Dermatol. 106:1096–1101 (1996).
Körner, A., S. Petrovic, and H. Höcker, Cell Membrane Lipids of Wool and Human Hair Form Liposomes, Tex. Res. J. 65:56–58 (1995).
Coderch, L., A. de la Maza, A. Pinazo, and J.L. Parra, Physicochemical Characteristics of Liposomes Formed with Internal Wool Lipids, J. Am. Oil Chem. Soc. 73:1713–1718 (1996).
Fonollosa, J., M. Martí, A. de la Maza, M. Sabés, J.L. Parra, and L. Coderch, Thermodynamic and Structural Aspects of Internal Wool Lipids, Langmuir 16:4808–4812 (2000).
de Pera, M., L. Coderch, J. Fonollosa, A. de la Maza, and J.L. Parra, Effect of Internal Wool Lipid Liposomes on Skin Repair, Skin Pharmacol. Appl. Skin Physiol. 13:188–195 (2000).
Moore, D.J., and M.E. Rerek, Recent Biophysical Studies of Ceramides and Their Role in Skin Barrier Function, Proc. Cos. Sci. Conf. (Barcelona) I:36–43 (2000).
Chen, H., R. Mendelsohn, M.E. Rerek, and D.J. Moore, Fourier Transform Infrared Spectroscopy and Differential Scanning Calorimetry Studies of Fatty Acid Homogeneous Ceramide 2, Biochim. Biophys. Acta 1468:293–303 (2000).
Gale, D.J., R.I. Logan, and D.E. Rivett, Detection of Desmosterol in the Internal Lipids of Wool Fibers, Text. Res. J. 57:539–542 (1987).
Alzaga, R., E. Pascual, P. Erra, and J.M. Bayona, Development of a Novel Supercritical Fluid Extraction Procedure for Lanolin Extraction from Raw Wool, Anal. Chim. Acta 381:39–48 (1999).
International Wool Textile Organisation, TWTO Test Method (E)-5-73 (E), Appendix B, IWTO, Brussels.
Leaver, I.H., D.M. Lewis, and D.J. Westmoreland, Analysis of Wool Lipids Using Thin-Layer Chromatography with Flame Ionization Detection, Text. Res. J. 58:593–600 (1988).
Logan, R.I., D.E. Rivett, D.J. Tucker, and H.F. Hudson, Analysis of the Intercellular and Membrane Lipids of Wool and Other Animal Fibres, 59:109–113 (1989).
Leeder, J.D., The Cell Membrane Complex and Its Influence on the Properties of the Wool Fibre, Wool Sci. Rev. 63:3–35 (1986).
Ponec, M., and A. Weerheim, Retinoids and Lipid Changes in Keratinocytes, Methods Enzymol. 190 (Retinoids, Pt. B):30–41 (1990).
Melnik, B.C., J. Hollmann, E. Erler, B. Verhoeven, and G. Plewig, Microanalytical Screening of All Major Stratum Corneum Lipids by Sequential High-Performance Thin-Layer Chromatography, J. Invest. Dermatol. 92:231–234 (1989).
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Coderch, L., Fonollosa, J., Martí, M. et al. Extraction and analysis of ceramides from internal wool lipids. J Amer Oil Chem Soc 79, 1215–1220 (2002). https://doi.org/10.1007/s11746-002-0630-3
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DOI: https://doi.org/10.1007/s11746-002-0630-3