Abstract
The aim of this study was to evaluate fibroblast growth factor (FGF)-23 serum levels and its tissue expression in patients with critical carotid artery stenosis (CAS). We selected 35 patients with critical CAS undergoing carotid thromboendoarterectomy. In each patient, FGF-23 serum levels were evaluated just prior to the surgery (t0) and 30 min (t1) thereafter. Moreover, macrophage cytokines were measured at baselines. Carotid artery specimens were used for immune histochemistry. On the basis of the histology, the patients were divided into 2 groups: A with complicated plaque and B with uncomplicated plaque. Twenty complicated plaques (57.14 %, group A,) and 15 uncomplicated (42.86 %, group B) were evaluated: calcifications were present in 16/20 (80 %) complicated plaques and in 6/15 (40 %) uncomplicated plaques. An inflammatory infiltrate was observed in 26/35 carotid samples: 18/26 (69.23 %) complicated and 8/26 (30.76 %) uncomplicated. FGF-23+ cells were present in 17/20 complicated (85 %) and in 8 uncomplicated (53 %) plaques. The double-staining immunofluorescence confirmed that macrophage cells (CD68+) were also positive for FGF-23 staining. Serum levels of FGF-23 were significantly higher in group A versus group B at t0 (p < 0.05) and t1 (p 0.0047). Moreover, in group A patients a significant increase of FGF-23 serum levels was observed at t1 in comparison with t0 (p 0.0011). Our results suggest that FGF-23 acts in the late phases of atherosclerotic disease and may potentially represent a marker of complications in critical CAS.
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Acknowledgments
This study was funded by the Università di Roma “La Sapienza”, Progetto di Ricerca di Università Anno: 2010-prot. C26A10ETWL
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The authors have no conflict of interest to declare, including specific financial interest or relationship with pharmaceutical companies, biomedical device manufacturers, or other corporations whose products or services are related to the subject matter of the article. Such relationships include employment by any industrial concern, ownership of stock, membership on a standing advisory council or committee, being on the board of directors or being publicly associated with the company of its products. Moreover, the authors declare that they have not received honoraria, fees, grants or funds from such corporations or individuals representing such corporations.
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Del Porto, F., Proietta, M., di Gioia, C. et al. FGF-23 levels in patients with critical carotid artery stenosis. Intern Emerg Med 10, 437–444 (2015). https://doi.org/10.1007/s11739-014-1183-3
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DOI: https://doi.org/10.1007/s11739-014-1183-3