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Culture media influenced laboratory outcomes but not neonatal birth weight in assisted reproductive technology

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Abstract

Whether the type of culture media utilized in assisted reproductive technology has impacts on laboratory outcomes and birth weight of newborns in in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) was investigated. A total of 673 patients undergoing IVF/ICSI and giving birth to live singletons after fresh embryo transfer on day 3 from Jan. 1, 2010 to Dec. 31, 2012 were included. Three types of culture media were used during this period: Quinn’s Advantage (QA), Single Step Medium (SSM), and Continuous Single Culture medium (CSC). Fertilization rate (FR), normal fertilization rate (NFR), cleavage rate (CR), normal cleavage rate (NCR), good-quality embryo rate (GQER) and neonatal birth weight were compared using one-way ANOVA and χ 2 tests. Multiple linear regression analysis was performed to determine the impact of culture media on laboratory outcomes and birth weight. In IVF cycles, GQER was significantly decreased in SSM medium group as compared with QA or CSC media groups (63.6% vs. 69.0% in QA; vs. 71.3% in CSC, P=0.011). In ICSI cycles, FR, NFR and CR were significantly lower in CSC medium group than in other two media groups. No significant difference was observed in neonatal birthweight among the three groups (P=0.759). Multiple linear regression analyses confirmed that the type of culture medium was correlated with FR, NFR, CR and GQER, but not with neonatal birth weight. The type of culture media had potential influences on laboratory outcomes but did not exhibit an impact on the birth weight of singletons in ART.

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Authors and Affiliations

  1. Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China

    Tai-lang Yin  (尹太郎), Yi Zhang  (张 怡), Sai-jiao Li  (李赛姣), Meng Zhao  (赵 萌), Jin-li Ding  (丁锦丽), Wang-ming Xu  (徐望明) & Jing Yang  (杨 菁)

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  1. Tai-lang Yin  (尹太郎)
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  2. Yi Zhang  (张 怡)
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  3. Sai-jiao Li  (李赛姣)
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  4. Meng Zhao  (赵 萌)
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  5. Jin-li Ding  (丁锦丽)
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  6. Wang-ming Xu  (徐望明)
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  7. Jing Yang  (杨 菁)
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Correspondence to Wang-ming Xu  (徐望明) or Jing Yang  (杨 菁).

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Yin, Tl., Zhang, Y., Li, Sj. et al. Culture media influenced laboratory outcomes but not neonatal birth weight in assisted reproductive technology. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 35, 932–937 (2015). https://doi.org/10.1007/s11596-015-1531-1

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  • DOI: https://doi.org/10.1007/s11596-015-1531-1

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