Abstract
Exposure to PM10 generated by biomass burning may reduce lung function and induce cytogenetic effects, especially in chronic obstructive pulmonary disease patients. This study investigated the frequency of DNA-damaged cells, cells with cytokinetic defect, and different types of cell death using a buccal micronucleus cytome assay. The correlations between each biomarker and lung function were investigated. The changes in these biomarkers associated with high pollutant levels (PM10 > 50 μg/m3) and low pollutant levels (PM10 < 50 μg/m3) were evaluated to explore whether PM10 exposure induced genotoxic damages and cytokinetic defects in COPD patients when the daily average PM10 concentration reached above 50 μg/m3. Fifty-eight COPD patients and 26 healthy subjects living in Chiang Dao district, Chiang Mai, Thailand, were recruited in this study. The results revealed that buccal cells with micronuclei (high vs low 1.09 ± 1.95 vs 0.29 ± 0.64 in COPD patients) and binucleated cells (high vs low 11.43 ± 18.68 vs 1.60 ± 1.31 and 7.77 ± 12.76 vs 1.00 ± 1.17 in COPD and healthy subjects, respectively) observed during the high pollutant period were more frequent than in the low pollutant period. Moreover, exposure to PM10 increased the risk of micronucleus induction in COPD patients 295.23-fold.
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The authors are grateful to Dr. Dale E. Taneyhill for proofreading the English.
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This research was supported by the Thailand Research Fund (TRF), Grant No. MRG5980190 and Faculty of Medicine, Chiang Mai University Research Fund, Grant No. 013/2560 and Grant No. 067/2559. The funding body had no role in the design and execution of this study or interpretation of the data.
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KW, CP, and TP conceived and planned the study. KW, SM, NP and WC contributed to sample collection and lung function measurement. KW and SM carried out the BMCyt assay. KW interpreted the results and performed data analysis. KW and RP performed statistical analysis. KW wrote the manuscript in consultation with CP, TP and WC.
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Wunnapuk, K., Pothirat, C., Manokeaw, S. et al. PM10-related DNA damage, cytokinetic defects, and cell death in COPD patients from Chiang Dao district, Chiang Mai, Thailand. Environ Sci Pollut Res 26, 25326–25340 (2019). https://doi.org/10.1007/s11356-019-05641-w
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DOI: https://doi.org/10.1007/s11356-019-05641-w