Abstract
Bovine calpastatin (CAST) gene polymorphisms have been associated with meat tenderness traits; however, little is known about how the CAST gene affects beef quality traits. In this study, 25 single nucleotide polymorphisms were detected in the CAST gene using polymerase chain reaction with single-strand conformation polymorphism and gene sequencing. Different preponderant genotypes were found at the corresponding gene locus. The E1-1, E1-2, and C3-1 loci were correlated with meat tenderness height and highly correlated with the fatty acid content and the amino acid content. The E4-2 locus was not correlated with meat tenderness, but it was correlated with cooking loss, brightness, and yellowness, among others. The CAST gene is a potential marker for these meat quality traits, but further research is required.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Juszczuk-Kubiak E, Wyszyńska-Koko J, Wicińska K, Rosochacki S (2008) A novel polymorphisms in intron 12 of the bovine calpastatin gene. Mol Biol Rep 35(1):29–35
Koohmaraie M (1996) Biochemical factors regulating the toughening and tenderization process of meat. Meat Sci 43:193–201
Allais S, Journaux L, Levéziel H, Payet-Duprat N, Raynaud P, Hocquette J, Lepetit J, Rousset S, Denoyelle C, Bernard-Capel C (2011) Effects of polymorphisms in the calpastatin and μ-calpain genes on meat tenderness in 3 French beef breeds. J Anim Sci 89(1):1–11
Li J, Zhang LP, Gan QF, Li JY, Gao HJ, Yuan ZR, Gao X, Chen JB, Xu SZ (2010) Association of CAST gene polymorphisms with carcass and meat quality traits in Chinese Commercial Cattle Herds. Asian Australas J Anim Sci 23(11):1405–1411
Schenkel F, Miller S, Jiang Z, Mandell I, Ye X, Li H, Wilton J (2006) Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. J Anim Sci 84(2):291–299
Xin J, Li-chun Z, Zhao-zhi L, Xiao-hui L, Hai-guo J, Chang-guo Y (2011) Association of polymorphisms in the Calpain I gene with meat quality traits in Yanbian yellow cattle of China. Asian Aust J Anim Sci 24(1):9–16
White S, Casas E, Wheeler T, Shackelford S, Koohmaraie M, Riley D, Chase C Jr, Johnson D, Keele J, Smith T (2005) A new single nucleotide polymorphism in CAPN1 extends the current tenderness marker test to include cattle of Bos indicus, Bos taurus, and crossbred descent. J Anim Sci 83(9):2001–2008
Chung E, Kim W, Kim Y, Han S (2000) DNA fingerprinting of Korean cattle using AFLP markers. Korean J Anim Sci 42(4):391–406
Oh JD, Lee J, Lee KW, Park KD, Cho BW, Jeon GJ, Lee HK, Kong HS (2010) Identification of polymorphisms in CAST gene associated with economic traits in Hanwoo (Bos taurus coreanae). J Life Sci 20(10):1498–1504
Seong J, Suh DS, Park KD, Lee HK, Kong HS (2012) Identification and analysis of MC4R polymorphisms and their association with economic traits of Korean cattle (Hanwoo). Mol biol rep 39(4):3597–3601
Choi B, Ahn B, Kook K, Sun S, Myung K, Moon S, Kim J (2002) Effects of feeding patterns and sexes on growth rate, carcass trait and grade in Korean native cattle. Asian Australas J Anim Sci 15(6):838–843
Lepage G, Roy C (1986) Direct transesterification of all classes of lipids in a one-step reaction. J Lipid Res 27(1):114–120
Chouinard P, Lévesque J, Girard V, Brisson G (1997) Dietary soybeans extruded at different temperatures: milk composition and in situ fatty acid reactions. J Dairy Sci 80(11):2913–2924
Zhou H, Hickford J, Gong H (2007) Polymorphism of the ovine calpastatin gene. Mol Cell Probes 21(3):242–244
Casas E, White S, Wheeler T, Shackelford S, Koohmaraie M, Riley D, Chase C Jr, Johnson D, Smith T (2006) Effects of calpastatin and μ-calpain markers in beef cattle on tenderness traits. J Anim Sci 84(3):520–525
Casas E, White S, Riley D, Smith T, Brenneman R, Olson T, Johnson D, Coleman S, Bennett G, Chase C Jr (2005) Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. J Anim Sci 83(1):13–19
Pringle T, Williams S, Lamb B, Johnson D, West R (1997) Carcass characteristics, the calpain proteinase system, and aged tenderness of Angus and Brahman crossbred steers. J Anim Sci 75(11):2955–2961
Chung H, Davis M, Hines H (2001) Genetic variants detected by PCR–RFLP in intron 6 of the bovine calpastatin gene. Anim Genet 32(1):53–54
Curi R, Chardulo L, Mason M, Arrigoni M, Silveira A, De Oliveira H (2009) Effect of single nucleotide polymorphisms of CAPN1 and CAST genes on meat traits in Nellore beef cattle (Bos indicus) and in their crosses with Bos taurus. Anim Genet 40(4):456–462
Morgan J, Wheeler T, Koohmaraie M, Savell J, Crouse J (1993) Meat tenderness and the calpain proteolytic system in longissimus muscle of young bulls and steers. J Anim Sci 71(6):1471–1476
Corva P, Soria L, Schor A, Villarreal E, Cenci MP, Motter M, Mezzadra C, Melucci L, Miquel C, Paván E (2007) Association of CAPN1 and CAST gene polymorphisms with meat tenderness in Bos taurus beef cattle from Argentina. Genetics Mol Biol 30(4):1064–1069
Phillips A, Means W, Kalchayanand N, McCormick R, Miller K (2000) Bovine placental protease specificity toward muscle connective tissue proteins. J Anim Sci 78(7):1861–1866
Taniguchi M, Utsugi T, Oyama K, Mannen H, Kobayashi M, Tanabe Y, Ogino A, Tsuji S (2004) Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese black cattle. Mamm Genome 15(2):142–148
Thompson R, Bautista FR, Cain R (1961) Effect of pre-irradiation heating temperatures, irradiation level, and storage time at 34 °F on the free amino acid composition of beef a, b. J Food Sci 26(4):412–415
Fuke S, Konosu S (1991) Taste-active components in some foods: a review of Japanese research. Physiol Behav 49(5):863–868
Huff-Lonergan E, Baas T, Malek M, Dekkers J, Prusa K, Rothschild M (2002) Correlations among selected pork quality traits. J Anim Sci 80(3):617–627
Morris C, Cullen N, Hickey S, Dobbie P, Veenvliet B, Manley T, Pitchford WS, Kruk ZA, Bottema CDK, Wilson T (2006) Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey × Limousin, Angus and Hereford-cross cattle. Anim Genet 37(4):411–414
Acknowledgments
This work was supported by the National High Technology Research and Development Project of China (No.2008AA101010) and National Key Technology R&D Program in the 11th Five year Plan of china (2008BADB2B01). The foundation (National High Technology Research and Development Project of China (No.2008AA101010) and National Key Technology R&D Program in the 11th Five year Plan of china (2008BADB2B01) will be replaced by National Natural Science Foundation of China (Analysis of the genes function with meat quality in Yanbian cattle, Grant No:31160442).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, Y.X., Jin, H.G., Yan, C.G. et al. Association of CAST gene polymorphisms with carcass and meat quality traits in Yanbian cattle of China. Mol Biol Rep 40, 1875–1881 (2013). https://doi.org/10.1007/s11033-012-2243-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-012-2243-2