Abstract
To investigate the genetic factors that affect fatty acid composition of beef, we compared the full-length bovine stearoyl-CoA desaturase (SCD) cDNA from 20 Japanese Black steers. Two types of the SCD gene with single nucleotide polymorphisms (SNPs) were observed in the ORF of SCD cDNA, in which an amino acid replacement from valine (type V) to alanine (type A) was predicted. We developed a method for genotyping these two SCD genes based on PCR-RFLP. We have classified 1003 Japanese Black carcasses into three genotypes, VV, VA, and AA, and compared fatty acid composition among them. The SCD type A gene contributed to higher MUFA percentage and lower melting point in intramuscular fat. The SCD genotype was not the only genetic factor contributing to fatty acid composition of Japanese Black steers, but the SCD genotype was considered one of the causes of genetic variation in fatty acid composition of Japanese Black steers. Transcription factors such as sterol regulatory element binding protein-1c (SREBP-1c) may account for the remaining part of the genetic variation in fatty acid composition.
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References
H Bene D Lasky JM Ntambi (2001) ArticleTitleCloning and characterization of the human stearoyl-CoA desaturase gene promoter: transcriptional activity by sterol regulatory elementbinding protein and repression by polyunsaturated fatty acids and cholesterol. Biochem Biophys Res Commun 284 1194–1198 Occurrence Handle10.1006/bbrc.2001.5102 Occurrence Handle1:CAS:528:DC%2BD3MXksVKjtrg%3D Occurrence Handle11414710
L Bernard C Leroux H Hayes M Gautier Y Chilliard et al. (2001) ArticleTitleCharacterization of the caprine stearoyl-CoA desaturase gene and its mRNA showing an unusually long 3′-UTR sequence arising from a single exon. Gene 281 53–61 Occurrence Handle10.1016/S0378-1119(01)00822-8 Occurrence Handle1:CAS:528:DC%2BD3MXptFelsLs%3D Occurrence Handle11750127
HP Cho MT Nakamura SD Clarke (1999) ArticleTitleCloning, expression, and nutritional regulation of the mammalian Δ6 desaturase. J Biol Chem 274 471–477 Occurrence Handle10.1074/jbc.274.1.471 Occurrence Handle1:CAS:528:DyaK1MXjvVajsg%3D%3D Occurrence Handle9867867
M Chung S Ha S Jeong J Bok K Cho M Baik Y Choi (2000) ArticleTitleCloning and characterization of bovine stearoyl CoA desaturase 1 cDNA from adipose tissues. Biosci Biotechnol Biochem 64 1526–1530 Occurrence Handle1:CAS:528:DC%2BD3cXlvFGqtLo%3D Occurrence Handle10945276
E Clemens V Arthaud R Mandigo W Woods (1973) ArticleTitleFatty acid composition of bulls and steers as influenced by age and dietary energy level. J Anim Sci 37 1326–1331 Occurrence Handle1:CAS:528:DyaE2cXhsVems7o%3D Occurrence Handle4763366
DS Falconer (1981) Introduction to Quantitative Genetics, 2nd ed. Longman Inc. New York 100–111
J Folch M Lees GHS Stanley (1957) ArticleTitleA simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226 487–509
A Haliassos JC Chomel S Grandjouan J Kruh JC Kaplan et al. (1989) ArticleTitleDetection of minority point mutations by modified PCR technique: a new approach for a sensitive diagnosis of tumor-progression markers. Nucleic Acids Res 17 8093–8099 Occurrence Handle1:CAS:528:DyaK3cXjslGn Occurrence Handle2573037
YC Kim JM Ntambi (1999) ArticleTitleRegulation of stearoyl-CoA desaturase genes: role in cellular metabolism and preadipocyte differentiation. Biochem Biophys Res Commun 266 1–4 Occurrence Handle10.1006/bbrc.1999.1704 Occurrence Handle1:CAS:528:DyaK1MXns1Sksbw%3D Occurrence Handle10581155
S Kumar K Tamura IB Jakobsen M Nei (2001) ArticleTitleMEGA2: Molecular evolutionary genetics analysis software. Bioinformatics 17 1244–1245 Occurrence Handle1:CAS:528:DC%2BD38XmtVCktQ%3D%3D Occurrence Handle11751241
IB Mandell JG Buchanan-Smith CP Campbell (1998) ArticleTitleEffects of forage vs grain feeding on carcass characteristics, fatty acid composition, and beef quality in Limousin-cross steers when time on feed is controlled. J Anim Sci 76 2619–2630 Occurrence Handle1:CAS:528:DyaK1cXntFWhu7o%3D Occurrence Handle9814903
SL Melton M Amiri GW Davis WR Backus (1982) ArticleTitleFlavor and chemical characteristics of ground beef from grass-, forage-, grain- and grain-finished steers. J Anim Sci 55 77–87 Occurrence Handle1:CAS:528:DyaL38XksFCmurk%3D
JM Ntambi SA Buhrow KH Kaestner RJ Christy E Sibley et al. (1988) ArticleTitleDifferentiation-induced gene expression in 3T3-L1 preadipocytes. J Biol Chem 263 17291–17300 Occurrence Handle1:CAS:528:DyaL1MXitVKrurw%3D Occurrence Handle2903162
T Oikawa T Sanehira K Sato Y Mizoguchi H Yamamoto et al. (2000) ArticleTitleGenetic parameters for growth and carcass traits of Japanese Black (Wagyu) cattle. Anim Sci 71 59–64
A Oka F Iwaki T Dohgo S Ohtagaki M Noda et al. (2002) ArticleTitleGenetic effects on fatty acid composition of carcass fat of Japanese Black Wagyu steers. J Anim Sci 80 1005–1011 Occurrence Handle1:CAS:528:DC%2BD38XmvV2it7s%3D Occurrence Handle12002306
PW O’Keefe GH Wellington LR Mattick JR Stouffer (1968) ArticleTitleComposition of bovine muscle lipids at various carcass locations. J Food Sci 33 188–192 Occurrence Handle1:CAS:528:DyaF1cXkt1Kis7c%3D
D Perry PJ Nicolls JM Thompson (1998) ArticleTitleThe effect of sire breed on the melting point and fatty acid composition of subcutaneous fat in steers. J Anim Sci 76 87–95 Occurrence Handle1:CAS:528:DyaK1cXlsVKjsQ%3D%3D Occurrence Handle9464888
AM Sessler N Kaur JP Palta JM Ntambi (1996) ArticleTitleRegulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acid in 3T3-L1 adipocytes. J Biol Chem 271 29854–29858 Occurrence Handle10.1074/jbc.271.47.29854 Occurrence Handle1:CAS:528:DyaK28XntFKku78%3D Occurrence Handle8939925
J Shanklin E Whittle BG Fox (1994) ArticleTitleEight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxyrase and xylene monooxygenase. Biochemistry 33 12787–12794 Occurrence Handle1:CAS:528:DyaK2cXmslGiurg%3D Occurrence Handle7947684
SB Smith A Yang TW Larsen RK Tume (1998) ArticleTitlePositional analysis of triacylglycerols from bovine adipose tissue lipids varying in degree of unsaturation. Lipids 33 197–207 Occurrence Handle1:CAS:528:DyaK1cXht1Oqu7s%3D Occurrence Handle9507242
P Strittmatter M Thiede CS Hackett J Ozols (1988) ArticleTitleBacterial synthesis of active rat stearyl-CoA desaturase lacking the 26-residue amino-terminal amino acid sequence. J Biol Chem 263 2532–2535 Occurrence Handle1:CAS:528:DyaL1cXhsVCltbo%3D Occurrence Handle2892838
M Taniguchi H Mannen K Oyama Y Shimakura H Watanabe et al. (2004) ArticleTitleDifferences in stearoyl-CoA desaturase mRNA levels between Japanese Black and Holstein cattle. Livestock Prod Sci (in press) . .
RJ Ward MT Travers SE Richards RG Vernon AM Salter et al. (1998) ArticleTitleStearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome. Biochim Biophys Acta 1391 145–156 Occurrence Handle10.1016/S0005-2760(97)00210-5 Occurrence Handle1:CAS:528:DyaK1cXhvFGrsrg%3D Occurrence Handle9554990
A Yang TW Larsen VH Powell RK Tume (1999a) ArticleTitleA comparison of fat composition of Japanese and long-term grain-fed Australian steers. Meat Sci 51 1–9
A Yang TW Larsen SB Smith RK Tume (1999b) ArticleTitleΔ9 desaturase activity in bovine subcutaneous adipose tissue of different fatty acid composition. Lipids 34 971–978 Occurrence Handle1:CAS:528:DyaK1MXmvVaht7g%3D
T Yoshimura K Namikawa (1985) ArticleTitleInfluence of breed, sex and anatomical location on lipid and fatty acid composition of bovine subcutaneous fat. Jpn J Zootech Sci 56 130–136 Occurrence Handle1:CAS:528:DyaL2MXkt1Sntrc%3D
M Zembayashi K Nishimura DK Lunt SB Smith (1995) ArticleTitleEffect of breed type and sex on the fatty acid composition of subcutaneous and intramuscular lipids of finishing steers and heifers. J Anim Sci 73 3325–3332 Occurrence Handle1:CAS:528:DyaK2MXps1eit7o%3D Occurrence Handle8586591
L Zhang LG Parismoo K Stenn SM Prouty (1999) ArticleTitleHuman stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochem J 340 255–264 Occurrence Handle10.1042/0264-6021:3400255 Occurrence Handle1:CAS:528:DyaK1MXjs12ks7g%3D Occurrence Handle10229681
L Zhang L Ge T Tran K Stenn SM Prouty (2001) ArticleTitleIsolation and characterization of the human stearoyl-CoA desaturase gene promoter: requirement of a conserved CCAAT cis element. Biochem J 357 183–193 Occurrence Handle10.1042/0264-6021:3570183 Occurrence Handle1:CAS:528:DC%2BD3MXlt1Ojurw%3D Occurrence Handle11415448
Acknowledgements
We thank Dr. Gregory S. Harper for helpful comments and Dr. Yasuhiko Shimakura for providing us with muscle samples for RNA extraction.
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Taniguchi, M., Utsugi, T., Oyama, K. et al. Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle . Mamm Genome 15, 142–148 (2004). https://doi.org/10.1007/s00335-003-2286-8
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DOI: https://doi.org/10.1007/s00335-003-2286-8