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Intercellular adhesion molecule-1 gene K469E polymorphism and ischemic stroke: a case-control study in a Chinese population

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Abstract

Background Intercellular adhesion molecule-1 (ICAM-1) was involved in the pathogenetic mechanisms responsible for ischemic stroke (IS). Population-based sample have revealed gene-gender interaction in blood pressure which is major risk for IS. We sought to evaluate whether ICAM-1 K469E polymorphism was involved in the causation of IS and whether it was different between female and male. Methods A 1:1 case-control study was conducted. The K469E polymorphism of ICAM-1 gene were analyzed by polymerase chain reaction (PCR) and restriction enzyme analysis in Chinese patients with IS (n = 309) and elderly subjects without IS (n = 309). Results ICAM-1 K469E polymorphism was significantly associated with IS. Interestingly, a further analysis stratified by sex found that there was significance between 469E genotypes and IS in female, but not in male. Multiple regression analysis revealed that ICAM-1 K469E polymorphism was still significantly associated with IS, compared with ICAM-1 KK genotype in all population (OR = 1.60, P = 0.030). Stratified by sex, EE combined EK was contributory factor to IS in female (OR = 3.03, P = 0.004), but not in male. After adjustment for confounding factors, the interaction between female and ICAM-1 EK/EE genotypes was found (OR = 3.54, P = 0.001). Conclusions It is suggested that the ICAM-1 469E allele may be important in the pathogenesis of ischemic stroke, especially in female but not in male.

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Abbreviations

ICAM-1:

Intercellular adhesion molecule-1

IS:

Ischemic stroke

RFLP:

Restriction fragment length polymorphism

TP:

Total protein

Alb:

Albumin

GLOB:

Globulin

HDL:

High density lipoprotein

CHOL:

Cholesterol

TG:

Triglyceride

BG:

Blood glucose

BMI:

Body mass index

WHR:

Waist circumference divided by the hip circumference

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Acknowledgements

This study was financially supported by a grant from the National Natural Science Foundation of China (30771852). We are grateful to all participants of this study. We also thank Dr. Yi Guo for his valuable comments and critical review of the manuscript and Feng Wang for his technical assistance.

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Authors and Affiliations

  1. Department of Epidemiology and Statistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, 430030, China

    Xiao Xia Li, Sheng Hong Han, Sheng Wei & Shao Fa Nie

  2. Department of Preventive Medicine, Mudanjiang Medical College, Mudanjiang, China

    Xiao Xia Li

  3. Department of Molecular Biology, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518020, China

    Jian Ping Liu, Jin Quan Cheng, Yi Jie Geng, Shi Tong Gao & Da Na Huang

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  1. Xiao Xia Li
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  2. Jian Ping Liu
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Correspondence to Shao Fa Nie.

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Xiao-Xia Li and Jian-Ping Liu have contributed equally.

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Li, X.X., Liu, J.P., Cheng, J.Q. et al. Intercellular adhesion molecule-1 gene K469E polymorphism and ischemic stroke: a case-control study in a Chinese population. Mol Biol Rep 36, 1565–1571 (2009). https://doi.org/10.1007/s11033-008-9351-z

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  • DOI: https://doi.org/10.1007/s11033-008-9351-z

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