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Determination of the survival of yeast and bacteria under the influence of gamma or UV radiation in the presence of some scavengers of OH radicals

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Abstract

Two new methods for quantitative determination of the role of scavengers of OH radicals in the radiation protection of various microorganisms were studied. The yeast and bacteria were irradiated with gamma or UVC radiations having quite different effect on living cells. Both methods based on the counting of colonies arising from surviving cells proved to be able to describe quantitatively the protective effect of scavengers. The described method may be helpful for study of the radiation protection of cells, radiation stability of DNA or the permeability of the cell membrane.

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Acknowledgments

This research has been performed under the auspices of Grant Agency of the Czech Republic, Project GA ČR 13-28721S.

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Authors and Affiliations

  1. Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University, 115 19, Prague 1, Czech Republic

    V. Múčka, J. Červenák, V. Čuba & P. Bláha

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  1. V. Múčka
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  2. J. Červenák
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  3. V. Čuba
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  4. P. Bláha
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Correspondence to V. Múčka.

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Múčka, V., Červenák, J., Čuba, V. et al. Determination of the survival of yeast and bacteria under the influence of gamma or UV radiation in the presence of some scavengers of OH radicals. J Radioanal Nucl Chem 304, 237–244 (2015). https://doi.org/10.1007/s10967-014-3696-7

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  • DOI: https://doi.org/10.1007/s10967-014-3696-7

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