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Red drum Sciaenops ocellatus growth and expression of bile salt-dependent lipase in response to increasing dietary lipid supplementation

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Abstract

Sciaenops ocellatus has a long history in aquaculture and many difficulties associated with its commercial culture have been addressed and successfully resolved; nevertheless, further research in lipid nutrition could address more comprehensive questions on the way these nutrients are utilized. The purpose of this study was to evaluate S. ocellatus growth and lipase gene expression in response to increasing dietary lipid supplementation. Four experimental diets were formulated to provide 3, 10, 16, or 23% lipid using menhaden fish oil. Twenty juveniles (mean initial weight 2.3 ± 0.1 g) were stocked per aquaria in a recirculating system; each diet was assigned to three aquaria and fed to fish for 6 weeks. At the end of the study, fish fed 3% of dietary lipid were significantly (P < 0.0001) smaller and showed significantly lower feed efficiency, condition factor, hepatosomatic index, and intraperitoneal fat than fish fed the other diets, but no differences were observed among fish fed 10, 16, or 23% lipid. A straight broken-line regression model for thermal growth coefficient provided an estimated value of 9.4% of dietary lipid as the optimal inclusion level. The bile salt-dependent lipase (BSDL) of red drum was 80.3 kDa. Relative gene expression of BSDL was significantly higher (P = 0.0007) in fish fed 10% lipid, with no differences among the other dietary treatments. Results provided could help monitor the metabolic status of farmed fish and contribute to optimize diet formulations based on maximum gene expression of BSDL for supplementation of dietary lipid.

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Acknowledgments

This research was partly supported by CONACYT (Consejo Nacional de Ciencia y Tecnologia), Mexico, for Drs. González-Félix (CONACYT Application 454912, Registry 312949) and Perez -Velazquez (CONACYT Application 454922, Registry 312944). The mention of trademarks or proprietary products does not constitute an endorsement of the product and does not imply its approval to the exclusion of other products that may also be suitable.

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Authors and Affiliations

  1. Department of Scientific and Technological Research, University of Sonora, Edificio 7-G, Blvd. Luis Donaldo Colosio s/n, e/Sahuaripa y Reforma, Col. Centro, C.P, 83000, Hermosillo, Sonora, Mexico

    Mayra L. González-Félix & Martin Perez-Velazquez

  2. Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX, 77843-2258, USA

    Delbert M. Gatlin III

  3. Fisheries and Mariculture Laboratory, Marine Science Institute, The University of Texas at Austin, Port Aransas, TX, 78373, USA

    Ken Webb

  4. College of Agriculture, Forestry and Natural Resource Management, Pacific Aquaculture and Coastal Resources Center, University of Hawaii at Hilo, Hilo, HI, 96720, USA

    Armando García-Ortega

  5. USDA, Food and Feed Safety Unit, Agricultural Research Service, College Station, TX, 77845, USA

    Michael Hume

  6. Department of Poultry Science, Texas A&M University, College Station, TX, 77845, USA

    Michael Hume

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  1. Mayra L. González-Félix
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  2. Delbert M. Gatlin III
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  3. Martin Perez-Velazquez
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  5. Armando García-Ortega
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Correspondence to Mayra L. González-Félix.

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González-Félix, M.L., Gatlin, D.M., Perez-Velazquez, M. et al. Red drum Sciaenops ocellatus growth and expression of bile salt-dependent lipase in response to increasing dietary lipid supplementation. Fish Physiol Biochem 44, 1319–1331 (2018). https://doi.org/10.1007/s10695-018-0523-z

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