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Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata)

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Abstract

In the present study, we report the isolation and characterization of seabream Sparus aurata pyloric caeca-duodenal lipase. Optimum activity was found at pH 8.5 and salinity of 50 mM NaCl. Lipase activity was sensitive to divalent ions, and extreme pH values (4, 5, and 12), being more stable at alkaline than acid pH. Optimum temperature was found at 50°C, but lipase was stable at temperatures below 40°C. Lipase has a bile salt sodium taurocholate requirement for increased activity. Gradient PAGE electrophoresis revealed the presence of four isoforms with apparent molecular masses of 34, 50, 68, and 84 KDa, respectively. Pyloric-duodenal lipase was able to hydrolyze emulsified alimentary oils. Results confirm the presence of true lipases in Sparus aurata digestive tract.

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Acknowledgments

The technical assistance of Antonia Barros, Mariam Hamdam, and Patricia Hinojosa is acknowledged. HN thanks the Government of Mexico, through CONACYT by for grant 000000000081074, and for financial of the CONACYT research project No. 0000000000084652 related to lipid digestibility, and to Universidad de Almeria for acceptance for research stay.

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Authors and Affiliations

  1. Centro de Investigaciones Biológicas del Noroeste, S.C., Mar Bermejo No. 195, Col. Playa Palo Santa Rita, 23000, La Paz, BCS, Mexico

    Héctor Nolasco

  2. Universidad de Almería, Carr. a Sacramento sn, 04120, La Cañada de San Urbano, Almería, Spain

    Francisco Moyano-López

  3. Centro Universitario de la Costa, Universidad de Guadalajara, Puerto Vallarta, Jalisco, Mexico

    Fernando Vega-Villasante

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  1. Héctor Nolasco
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Correspondence to Héctor Nolasco.

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Nolasco, H., Moyano-López, F. & Vega-Villasante, F. Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata). Fish Physiol Biochem 37, 43–52 (2011). https://doi.org/10.1007/s10695-010-9414-7

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