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Discovery of QTL for stay-green and heat-stress in barley (Hordeum vulgare) grown under simulated abiotic stress conditions

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Abstract

This study maps genomic regions associated with terminal heat- and drought-stress tolerance in barley (Hordeum vulgare L.). One hundred lines were randomly sampled from a ND24260 × Flagship doubled haploid population and evaluated for stay-green (SG) expression. SG expression including that of parental controls and commercial check varieties was evaluated in two controlled environments; one simulating terminal heat-stress, the other terminal water-stress. During grain-fill the greenness of the spikes (S), flag leaf (FL) and the first leaf under the flag leaf (FL-1) were phenotyped; visually (using a 0–9 scale) and via single-photon avalanche diode measurements. From the visual assessments, the green leaf area of the plant was determined, by using the difference in green area of the S and FL. Composite interval mapping detected 10 quantitative trait loci (QTL) for SG, positioned on chromosomes 3H, 4H, 5H, 6H and 7H; six of which were associated with terminal heat-stress and four with terminal water-stress. None were co-located with previously reported barley stress-response QTL and thus represent novel barley QTL. Although novel, some SG QTL mapped near chromosomal regions previously reported; such as the two heat-stress QTL mapped to bPb-5529 on 5H, adjacent to QTL reported for root length and root-shoot ratio. Detection of SG QTL in barley grown under simulated heat- and water-stressed conditions offers the potential of high through put screening for these traits. If confirmed in field trials, these genomic regions will be candidates for barley breeding programs targeting improved abiotic stress tolerance via marker-assisted selection.

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Abbreviations

SG:

Stay-green

MAS:

Marker-assisted selection

GS:

Green spike

GFL:

Green flag leaf

GFL-1:

Green first leaf under the flag leaf

LAUG:

Leaf area under green

SP:

SPAD (Single-photon avalanche diode)

DH:

Double haploid

CIM:

Composite interval mapping

DArT:

Diversity Array Technology

LOD:

Logarithm of odds

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Acknowledgments

The authors thank and acknowledge the support of the 1000-Talents Program of the Chinese Government’s Foreign Experts Bureau, and the Grain Research and Development Corporation for a Ph.D. top-up scholarship.

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Authors and Affiliations

  1. Queensland Alliance for Agriculture and Food Sciences, The University of Queenslan, St Lucia, QLD, 4072, Australia

    Peter W. Gous, Lee Hickey & Glen P. Fox

  2. Queensland Alliance for Agriculture and Food Sciences, Leslie Research Facility, The University of Queensland, Toowoomba, QLD, 4350, Australia

    Jack T. Christopher

  3. Department of Agriculture, Fisheries and Forestry, Hermitage Research Facility, Warwick, QLD, 4370, Australia

    Jerome Franckowiak

  4. Department of Agronomy and Plant Genetics, University of Minnesota Twin Cities, St Paul, MN, 55108, USA

    Jerome Franckowiak

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  1. Peter W. Gous
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  2. Lee Hickey
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  3. Jack T. Christopher
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  4. Jerome Franckowiak
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  5. Glen P. Fox
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Correspondence to Glen P. Fox.

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Gous, P.W., Hickey, L., Christopher, J.T. et al. Discovery of QTL for stay-green and heat-stress in barley (Hordeum vulgare) grown under simulated abiotic stress conditions. Euphytica 207, 305–317 (2016). https://doi.org/10.1007/s10681-015-1542-9

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