Abstract
Presence of microcystin (MC), a predominant freshwater algal toxin and a suspected liver carcinogen, in Florida’s freshwaters poses serious health threat to humans and aquatic species. Being recalcitrant to conventional physical and chemical water treatment methods, biological methods of MC removal is widely researched. Water samples collected from five sites of Lake Okeechobee (LO) frequently exposed to toxic Microcystis blooms were used as inoculum for enrichment with microcystin LR (MC-LR) supplied as sole C and N source. After 20 days incubation, MC levels were analyzed using high performance liquid chromatography (HPLC). A bacterial consortium consisting of two isolates DC7 and DC8 from the Indian Prairie Canal sample showed over 74% toxin degradation at the end of day 20. Optimal temperature requirement for biodegradation was identified and phosphorus levels did not affect the MC biodegradation. Based on 16S rRNA sequence similarity the isolate DC8 was found to have a match with Microbacterium sp. and the DC7 isolate with Rhizobium gallicum (AY972457).
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Audubon of Florida (2005) Lake Okeechobee: A synthesis of information and recommendations for its restoration. In: Gray PN, Farrell CJ, Kraus ML, Gromnicki AH (eds) Audubon of Florida, Miami, p 106
Burns J (2007) Toxic cyanobacteria in Florida waters. In: Kenneth Hudnell H (ed) Proceedings of the interagency, international symposium on cyanobacterial harmful algal blooms, Chapter 5. Adv Exp Med Biol 619:127–137
Christofferson K, Lyck S, Winding A (2002) Microbial activity and bacterial community structure during degradation of microcystins. Aquat Microb Ecol 27:125–136
Codd GA, Ward CJ, Bell SG (1997) Cyanobacterial toxins: occurrence, modes of action, health effects and exposure routes. Arch Toxicol Suppl 19:399–410
Cousins IT, Bealing DJ, James HA, Sutton A (1996) Biodegradation of MC-LR by indigenous mixed bacterial populations. Water Res 30:481–485
Dawson RM (1998) Toxicology of microcystins. Toxicon 36:953–962
Edwards C, Lawton LA (2009) Bioremediation of cyanotoxins. In: Laskin AI, Gadd GM, Sariaslani S (eds) Advances in applied microbiology. Academic Press, New York, pp 109–129
Falconer IR (1991) Tumor promotion and liver injury caused by oral consumption of cyanobacteria. Environ Toxicol Water Qual 6:177–184
Figueiredo DR, Azeiteiro UM, Esteves SM, Goncalves FJM, Pereira MJ (2004) Microcystin producing blooms—a serious global public health issue. Ecotoxicol Environ Saf 59:151–163
Fischer WJ, Hitzfeld BC, Tencalla F, Eriksson JE, Mikhailov A, Dietrich DR (2000) Microcystin-LR toxicodynamics, induced pathology, and immunohistochemical localization in livers of blue-green algae exposed rainbow trout (Oncorhynchus mykiss). Toxicol Sci 54:365–373
Fleming LE, Stephan W (2001) Blue green algae, their toxins and public health issues. Report to the Florida Harmful Algal Bloom Taskforce. NIEHS Marine and Freshwater Biomedical Sciences Center, University of Miami, Miami
Fleming LE, Rivero C, Burns J, Williams C, Bean JA, Shea KA, Shin J (2002) Blue green algal (cyanobacterial) toxins, surface drinking water and liver cancer in Florida. Harmful Algae 1:157–168
Harada KI, Matsuura K, Suzuki M, Watanabe MF, Oishi S, Dahlem AM, Beasley VR, Carmichael WW (1990) Isolation and characterization of the minor components associated with microcystins LR and RR in the cyanobacterium (blue-green algae). Toxicon 28:55–64
Harada KI, Imanishi S, Kato H, Masayoshi M, Ito E, Tsuji K (2004) Isolation of Adda from microcystin-LR by microbial degradation. Toxicon 44:107–109
Ho L, Gaudieux AL, Fanok S, Newcombe G, Humpage AR (2007) Bacterial degradation of microcystin toxins in drinking water eliminates their toxicity. Short communication. Toxicon 50:438–441
Jones GJ, Orr PT (1994) Release and degradation of microcystin following algicide treatment of a Microcystis aeruginosa bloom in a recreational lake, as determined by HPLC and protein phosphatase inhibition assay. Water Res 28:871–876
Jones GJ, Bourne DG, Blakeley RL, Doelle H (1994) Degradation of the cyanobacterial hepatotoxin microcystin by aquatic bacteria. Nat Toxins 2:228–235
Kenefick SL, Hrudey SE, Peterson HG, Prepas EE (1993) Toxin release from Microcystis aeruginosa after chemical treatment. Water Sci Technol 27:433–440
Lahiti K, Hiisverta L (1989) Removal of cyanobacterial toxins in water treatment processes—review of studies conducted in Finland. Water Supply 7:149–154
Lam AKY, Fedorak PM, Prepas EE (1995) Biotransformation of the cyanobacterial hepatotoxin microcystin LR, as determined by HPLC and protein phosphatase bioassay. Environ Sci Technol 29:242–246
Laura D, De Socio G, Frassanito R, Rotilio D (1996) Effects of atrazine on Ochrobactrum anthropi membrane fatty acids. Appl Environ Microbiol 62:2644–2646
Lemes GAF, Kersanach R, Pinto LS, Dellagostin OA, Yunes JS, Mattheinsen A (2007) Biodegradation of microcystins by aquatic Burkholderia sp. from a south Brazilian coastal lagoon. Ecotoxicol Environ Saf 69:358–365
Matthiensen A, Beattie KA, Yunes JS, Kaya K, Codd GA (2000) [DLeu] microcystin-LR, from the cyanobacterium Microcystis RST 9501 and from a Microcystis bloom in the Patos Lagoon estuary, Brazil. Phytochemistry 55:383–387
Metcalf JS, Codd GA (2004) Cyanobacterial toxins in the water environment. A review of current knowledge. Foundation for Water Research. University of Dundee, Marlow, Bucks
Newcombe G, Cook D, Brooke S, Ho L, Slyman N (2003) Treatment options for microcystin toxins: similarities and differences between variants. Environ Toxicol 24:299–308
Park HD, Sasaki Y, Maruyama T, Yanagisawa E, Hiraishi A, Kato K (2001) Degradation of cyanobacterial hepatotoxin microcystin by a new bacterium isolated from a hypertrophic lake. Environ Toxicol 16:337–343
Pathmalal MM, Edwards C, Singh BK, Lawton LA (2009) Isolation and identification of novel microcystin-degrading bacteria. Appl Environ Microbiol 75:6924–6928
Pflugmacher S, Codd GA, Steinberg CEW (1999) Effects of the cyanobacterial toxin microcystin-LR on detoxication enzymes in aquatic plants. Environ Toxicol 14:111–115
Phlips EJ, Bledsoe E, Cichra M, Badylak S (2003) The distribution of potentially toxic cyanobacteria in Florida. In: Johnson D, Harbison RD (eds) Proceedings of health effects of exposure to cyanobacterial toxins: State of the Science. Florida Department of Health and the Center for Disease Control. St. Petersburg, Florida
Robinson NA, Pace J, Matson CF, Miura GA, Lawrence WB (1991) Characterization of chemically titrated microcystin-LR and its distribution in mice. Toxicol In Vitro 5:341–345
Ruiz-Arias A, Juarez-Ramirez C, de los Cobos-Vasconcelos D, Ruiz-Ordaz N, Salmeron-Alcocer A, Ahuatzi-Chacon D, Galindez-Mayer J (2010) Aerobic biodegradation of a sulfonated phenylazonaphthol dye by a bacterial community immobilized in a multistage packed-bed BAC reactor. Appl Biochem Biotechnol 162:1689–1707
SFWMD (South Florida WATER Management District) Lake Okeechobee: Algal Bloom Monitoring Program. http://www.sfwmd.gov/portal/page/portal/pg_grp_sfwmd_watershed/pg_sfwmd_watershed_inlake?_piref2294_4946961_2294_4946960_4946960.tabstring=tab20940638&_piref2294_4946961_2294_4946960_49469602294_20940817_2294_4946514_20940639.tabstring=tab20940808. Accessed 9 Oct 2008
Steidinger KA, Landsberg JH, Tomas CR, Burns JW (1999) Harmful algal blooms in Florida. Florida Marine Research Institute, St. Petersburg, FL. Unpublished technical report submitted to the Florida Harmful Algal Bloom Task Force
Tindall BJ, Rossello-Mora R, Busse HJ, Ludwig W, Kampfer P (2010) Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266
Tsuji K, Asakawa M, Anzai Y, Sumino T, Harada K (2006) Degradation of microcystin using immobilized microorganisms isolated in a eutrophic lake. Chemosphere 65:1117–1124
Valeria AM, Ricardo EJ, Stephan P, Alberto WD (2006) Degradation of microcystin-RR by Sphingomonas sp. CBA4 isolated from San Roque reservoir (Cordoba—Argentina). Biodegradation 17:447–455
Wei G, Yua J, Zhua Y, Chena W, Wanga L (2008) Characterization of phenol degradation by Rhizobium sp. CCNWTB 701 isolated from Astragalus chrysopteru in mining tailing region. J Hazard Mater 151:111–117
Williams CD, Aubel MT, Chapman AD, Aiuto PE (2007) Identification of cyanobacterial toxins in Florida’s Freshwater systems. Lakes Reserv Manag 23:144–152
Woo PCY, Teng JLL, Wu JKL, Leung FPS, Herman Tse H, Fung AMY, Lau SKP, Yuen K (2009) Guidelines for interpretation of 16S rRNA gene sequence-based results for identification of medically important aerobic Gram-positive bacteria. J Med Microbiol 58:1030–1036
World Health Organization, Geneva (1998) Guidelines for drinking-water quality, 2nd edn. Addendum to vol 2. Health criteria and other supporting information, pp 95–110
World Health Organization (1999) Cyanobacterial toxins. In: Chorus I, Bartram J (eds) Toxic cyanobacteria in water: a guide to their public health consequences, monitoring and management.
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We thank the ARCH Core Facility at Florida International University, Miami for providing MC-LR and helping with the HPLC analysis.
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Ramani, A., Rein, K., Shetty, K.G. et al. Microbial degradation of microcystin in Florida’s freshwaters. Biodegradation 23, 35–45 (2012). https://doi.org/10.1007/s10532-011-9484-y
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DOI: https://doi.org/10.1007/s10532-011-9484-y