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Effect of light-emitting diode (ʎ 627 nm and 945 nm ʎ) treatment on first intention healing: Immunohistochemical analysis

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Abstract

Collagen I is not only responsible for maintaining the integrity of most tissues due to its mechanical properties, but also for its active participation in the functionality of tissues because of its interaction with cells present in the extracellular matrix. The synthesis of collagen begins with tissue injury and remains until the end of the healing process. The use of non-coherent light for healing processes is still understudied. This procedure stands out as a biostimulation method for tissue repair, which increases local circulation, cell proliferation, and collagen synthesis. This study sought to quantify collagen I in the healing process after the treatment of wounds with the light-emitting diode (LED) treatment. The histologic analysis with tissue samples stained with picrosirius red showed a statistical difference between the positive controls, LED 627 and LED 945 nm groups; the group treated with LED 627 nm showed a predominance of mature collagen. The immunohistochemical analysis showed a statistically significant high concentration of collagen I in the LED 945 nm group. The irradiation of wounds with the higher wavelength (945 nm) used in the study produced the best activity of collagen I formation in experimental model.

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Authors and Affiliations

  1. Laboratory of Neuroanatomy and Neurophysiology, Universidade Estadual do Centro-Oeste—UNICENTRO Rua Vicente Machado, 3459, CEP: 85035-180, Guarapuava, Paraná, Brazil

    Ivo Ilvan Kerppers

  2. Institute of Biomedical Engineering, Universidade Camilo Castelo (Unicastelo), Estrada Dr. Altino Bondesan, 500, CEP:12247-016, São José dos Campos, SP, Brazil

    Carlos José de Lima, Adriana Barrinha Fernandes & Antonio Balbin Villaverde

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  1. Ivo Ilvan Kerppers
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  2. Carlos José de Lima
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  3. Adriana Barrinha Fernandes
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  4. Antonio Balbin Villaverde
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Correspondence to Ivo Ilvan Kerppers.

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Kerppers, I.I., de Lima, C.J., Fernandes, A.B. et al. Effect of light-emitting diode (ʎ 627 nm and 945 nm ʎ) treatment on first intention healing: Immunohistochemical analysis. Lasers Med Sci 30, 397–401 (2015). https://doi.org/10.1007/s10103-014-1668-3

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  • DOI: https://doi.org/10.1007/s10103-014-1668-3

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