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Anticancer effect of lipids partially purified from Pacific oyster, Crassostrea gigas on PC3 cells

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Abstract

The objective of this study was to determine the anticancer effects of the Pacific oyster (Crassostrea gigas) in vitro. For this study, the lipid extracts of C. gigas were prepared using several organic solvents: methanol, chloroform, hexane, methanol:chloroform=1:1, chloroform: hexane=1:1. The anticancer activity of the extracts was evaluated using cell cycle and apoptosis assays analyzed by a flow cytometry. Of all the extracts, the hexane extracts exhibited the highest anticancer activity compared to the other extracts. The hexane extracts were further separated and purified using thin layer chromatography. The final isolated lipid compounds were identified their components as palmitic acid, margaric acid, and stearic acid. These results indicate that this combination of lipids effectively inhibit in vitro human prostate tumor growth by inducing apoptosis of cancer cells.

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Authors and Affiliations

  1. Department of Biotechnology, Konkuk University, Chungju, Chungbuk, 380-701, Korea

    Eun-Kyung Kim, Yon-Suk Kim, Seung-Jae Lee & Pyo-Jam Park

  2. Faculty of Applied Marine Science, Cheju National University, Jeju, Jeju, 690-756, Korea

    You-Jin Jeon

  3. Department of Internal Medicine, Konkuk University, Chungju, Chungbuk, 380-701, Korea

    Jae Dong Lee

  4. Department of Biotechnology and Bio-Environmental Technology (BET) Research Institute, Chung-Ang University, Anseong, Gyeonggi, 456-756, Korea

    Tae-Il Son

  5. Division of Food Science and Aqualife Medicine, Chonnam National University, Yeosu, Jeonnam, 550-749, Korea

    Chang-Bum Ahn

  6. Department of Food Science & Biotechnology, Kunsan National University, Gunsan, Jeonbuk, 573-701, Korea

    Yong-Tae Kim

  7. Korean Nokyong Research Center, Konkuk University, Chungju, Chungbuk, 380-701, Korea

    Sang-Ho Moon & Byong-Tae Jeon

  8. Department of Biochemistry & Molecular Biology, University of Calgary, Calgary, T2N4N1, Canada

    Sung-Woo Kim

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  1. Eun-Kyung Kim
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  11. Sung-Woo Kim
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Correspondence to Pyo-Jam Park.

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Kim, EK., Kim, YS., Lee, SJ. et al. Anticancer effect of lipids partially purified from Pacific oyster, Crassostrea gigas on PC3 cells. Food Sci Biotechnol 19, 213–217 (2010). https://doi.org/10.1007/s10068-010-0029-7

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  • DOI: https://doi.org/10.1007/s10068-010-0029-7

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