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In vitro anti-proliferative activities of the sterols and fatty acids isolated from the Persian Gulf sponge; Axinella sinoxea

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Abstract

Purpose

Marine sponges are rich sources of anticancer metabolites. Axinella sinoxea is a less studied sponge, found in the Larak Island’s waters, of the Persian Gulf. In the present study, we have explored the cytotoxic properties and chemical constituents of A. sinoxea.

Methods

Repeated silica gel flash column chromatography of methanol extract of the Axinella sinoxea sponge, yielded fatty acid and sterol fractions. These fractions were analyzed by GC-MS and their anti-proliferative activities were evaluated by MTT assay against three human cancer cell lines including MOLT-4, MCF-7 and HT-29 as well as NIH/3 T3 fibroblast cells. The sterol-rich fractions were pooled and purified by HPLC and its sub fractions’ cytotoxic activities were evaluated by MTT assay against MOLT-4 and NIH/3 T3 cells.

Results

The GC-MS spectral analysis of a fraction eluted with hexane: diethyl ether (90: 10), resulted in the identification of twelve fatty acids, including five linear chain saturated fatty acids; tetrdecanoic acid (1), pentadecanoic acid (3), hexadecanoic acid (5), heptadecanoic acid (7), and octadecanoic acid (10); one branched chain isoprenoid fatty acid, 4,8,12-trimethyltridecanoic acid (2); four monoenoic fatty acids; 9-hexadecenoic acid (4), 7-methyl-6-hexadecanoic acid (6), 9-octadecenoic acid (8) and 11-octadecenoic acid (9) and two polyunsaturated fatty acids; 5,8,11,14-eicosatetraenoic acid (11) and 4,7,10,13,16,19-docosahexaenoic acid (12). Spectral analysis of a non-polar fraction eluted with hexane: diethyl ether (85: 15), resulted in the identification of eight steroids including: cholesta-5,22-dien-3β-ol (13), cholest-5-en-3β-ol (14), ergosta-5,22-dien-3β-ol (15), ergost-5-en-3β-ol (16), stigmasta-5,22-dien-3β-ol (17), γ-sitosterol (18), 33-norgorgosta-5,24(28)-dien-3β-ol (19) and stigmasta-5,24(28)-dien-3β-ol (20). Fatty acids-containing fraction was active against HT-29 cell line with IC50 26.52 ± 8.19 μg/mL, while the steroids-rich fraction was active against the three above mentioned cell lines with IC50 values of 1.20 ± 0.24, 4.12 ± 0.40 and 2.47 ± 0.31 μg/mL, respectively. All of the above-mentioned fractions and sub-fractions were inactive (IC50s > 50 μg/mL) when assayed against normal fibroblast cells.

Conclusion

The present study suggests A. sinoxea as a potential natural source of cancer chemotherapeutics.

Cytotxic constituents of Axinella sinoxea.

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Acknowledgements

We would like to thanks the Vice-Provosts for Researches of University of Hormozgan, Bandar Abbas and Shiraz University of Medical Sciences, for their financial support of this project. The authors wish to thank Mr. H. Argasi at the Research Consultation Center (RCC) of Shiraz University of Medical Sciences for his valuable assistance in editing this manuscript.

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Authors and Affiliations

  1. Department of Marine Biology, Faculty of Marine Sciences and Technology, University of Hormozgan, Bandar Abbas, Iran

    Fatemeh Heidary Jamebozorgi & Morteza Yousefzadi

  2. Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Zip: 71348-53734, Iran

    Fatemeh Heidary Jamebozorgi, Omidreza Firuzi & Amir Reza Jassbi

  3. Persian Gulf and Oman Sea Ecological Research, Agricultural Research, Education and Extension Organization, Iranian Fisheries Research Institute, Bandar Abbas, Iran

    Meliika Nazemi

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  1. Fatemeh Heidary Jamebozorgi
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Heidary Jamebozorgi, F., Yousefzadi, M., Firuzi, O. et al. In vitro anti-proliferative activities of the sterols and fatty acids isolated from the Persian Gulf sponge; Axinella sinoxea. DARU J Pharm Sci 27, 121–135 (2019). https://doi.org/10.1007/s40199-019-00253-8

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  • DOI: https://doi.org/10.1007/s40199-019-00253-8

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