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Molecular identification of Schoenoplectiella species (Cyperaceae) by use of microsatellite markers

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Abstract

Over the past few decades, use of molecular markers for species delimitation has drastically increased. Schoenoplectiella Lye has been recognized as a taxonomically difficult genus because of its morphological simplicity and frequent interspecific hybridization. The main objective of this study was to clarify the taxonomic identities of eight Schoenoplectiella species by use of molecular markers. We also evaluated the genetic relationships among S. × trapezoidea, known as a natural hybrid, and its close relatives. We used six microsatellite markers for 44 individuals from 31 natural populations of eight Schoenoplectiella species in South Korea. Six microsatellite marker combinations generated 59 amplification-detectable bands, of which 33 were specifically detected in one or more individuals of each species. Cluster analysis revealed that the grouping was consistent with the taxonomically recognized species. Our results do not support the hybrid origin of S. × trapezoidea. Rather, they suggest that this species is more closely related to S. hotarui. The informative microsatellite markers enabled us to clarify the distinctions among Schoenoplectiella species from South Korea and to identify the genetic relationships among these species. The molecular signatures found suitable for accurate identification of Schoenoplectiella species can be reliably used for studies of the phylogeny and evolution of this genus.

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Acknowledgments

The authors thank two anonymous reviewers for their critical comments and suggestions to improve our manuscript. This work was supported by the National Research Foundation of Korea (grant no. NRF-2009-0088457) from the Ministry of Education, Science and Technology (MEST) of the Republic of Korea.

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Author notes

  1. Changkyun Kim

    Present address: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, Yunnan, People’s Republic of China

Authors and Affiliations

  1. Department of Biological Science, Ajou University, Suwon, 443-749, South Korea

    Changkyun Kim, Jongduk Jung & Hong-Keun Choi

Authors
  1. Changkyun Kim
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  2. Jongduk Jung
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Correspondence to Hong-Keun Choi.

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Appendix

Appendix

List of samples of Schoenoplectiella species in South Korea used in the SSR marker analysis. All voucher specimens are deposited in AJOU. Information is presented in the following order: Species name––Locality, voucher specimen (abbreviation of OTU).

Schoenoplectiella hotarui (Ohwi) J. Jung and H.-K. Choi––Mt. Chilbo, Ansan-city, Gyeonggi-do, J. Jung 807019 (SHO1); Cheonjin lake, Goseong-gun, Gangwon-do, J. Jung 808329 (SHO2), J. Jung 808333 (SHO4), J. Jung 909130 (SHO5), J. Jung 909131 (SHO6); Seongdong-ri, Bolyeong-city, Chungcheongnam-do, J. Jung 809003 (SHO3).

S. juncoides (Roxb.) Lye––Daechi-ri, Geunam-myeon, Hadong-gun, Gyeongsangnam-do, H.R. Na 80257 (SJU1), J. Jung 908023 (SJU8); Homaesil-dong, Suwon-city, Gyeonggi-do, J. Jung 807020 (SJU3); Jangpan-ri, Cheoncheon-myeon, Jangsu-gun, Jeollabuk-do, J. Jung 807144 (SJU4); Gyera-ri, Doam-myeon, Gangjin-gun, Jeollanam-do, J. Jung 808051 (SJU5); Nonmi-ri, Hanam-myeon, Hwacheon-gun, Gangwon-do, J. Jung 808301 (SJU6); Songwon reservoir, Songwon-ri, Jingyo-myeon, Hadong-gun, Gyeongsangnam-do, J. Jung 908032 (SJU7).

S. komarovii (Roshev.) J. Jung and H.-K. Choi––Nonmi-ri, Hanam-myeon, Hwacheon-gun, Gangwon-do, H.R. Na s.n. (SKO1); Daecheong lake, Sangjang-ri, Munei-myeon, Cheongwon-gun, Chungcheongbuk-do, S.M. Yun s.n. (SKO2, SKO3).

S. lineolata (Franch. and Sav.) J. Jung and H.-K. Choi––Imcheon reservoir, Imcheon-ri, Gangjin-eup, Gangjin-gun, Jeollanam-do, J. Jung 808041 (SLI1); Nonmi-ri, Hanam-myeon, Hwacheon-gun, Gangwon-do, J. Jung 808306 (SLI2); Owol-ri, Chuncheon city, Gangwon-do, J. Jung 808310 (SLI3); Deudulbangjuk, Mosan-dong, Jeoncheon city, Chungcheongbuk-do, J. Jung 907445 (SLI4); Bonggang-ri, Bonggang-myeon, Gwangyang city, Jeollanam-do, J. Jung 908045 (SLI5).

S. mucronata (L.) J. Jung and H.-K. Choi––Sanho-ri, sanho-eup, Yeongam-gun, Jeollanam-do, J. Jung 808028 (SMU1); Imcheon-ri, Gangjin-eup, Gangjin-gun, Jeollanam-do, J. Jung 808035 (SMU2); Giljeong-ri, Gilsang-myeon, Ganghwa-gun, Incheon, J. Jung 809002 (SMU3); Songwon reservoir, Songwon-ri, Jingyo-myeon, Hadong-gun, Gyeongsangnam-do, J. Jung 908034 (SMU4); Jukrim reservoir, Jukrim-ri, Sora-myeon, Yeosu city, Jeollanam-do, J. Jung 908052 (SMU5).

S. × trapezoidea (Koidz.) J. Jung and H.-K. Choi––Cheonjin lake, Goseong-gun, Gangwon-do, J. Jung 808331 (STZ1), J. Jung 808331 (STZ2), J. Jung 909132 (STZ3), J. Jung 909132 (STZ4), J. Jung 909132 (STZ5), J. Jung 909133 (STZ6).

S. triangulata (Roxb.) J. Jung and H.-K. Choi––Suseo-ri, Gunui-eup, Gunui-gun, Gyeongsangbuk-do, H.R. Na 80263 (STR1); Geumyang-ri, Hapcheon-eup, Hapcheon-gun, Gyeongsangnam-do, H.R. Na 80303 (STR2); Seobu reservioir, Yongsu-ri, Hangyeong-myeon, Jeju city, Jeju-do, J. Jung 807066 (STR3); Haengwon-ri, Jangheung-eup, Jangheung-gun, Jeollanam-do, J. Jung 807140 (STR4); Nonmi-ri, Hanam-myeon, Hwacheon-gun, Gangwon-do, J. Jung 808302 (STR5); Cheonjin lake, Goseong-gun, Gangwon-do, J. Jung 808327 (STR6), J. Jung 909128 (STR7), H. -K. Choi s.n. (STR9); Gangseondal reservoir, Yongjeong-ri, Sangha-myeon, Gochang-gun, Jeollabuk-do, J. Jung 909160 (STR8).

S. wallichii (Nees) Lye––Bangsan-ri, Daegang-myeon, Namwon city, Jeollabuk-do, H. -K. Choi s.n. (SWA1, SWA02, SWA03).

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Kim, C., Jung, J. & Choi, HK. Molecular identification of Schoenoplectiella species (Cyperaceae) by use of microsatellite markers. Plant Syst Evol 298, 811–817 (2012). https://doi.org/10.1007/s00606-012-0592-x

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