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An electrochemical immunosensor based on interdigitated array microelectrode for the detection of chlorpyrifos

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Abstract

An electrochemical immunosensor based on interdigitated array microelectrodes (IDAMs) was developed for sensitive, specific and rapid detection of chlorpyrifos. Anti-chlorpyrifos monoclonal antibodies were orientedly immobilized onto the gold microelectrode surface through protein A. Chlorpyrifos were then captured by the immobilized antibody, resulting in an impedance change in the IDAMs surface. Electrochemical impedance spectroscopy was used in conjunction with the fabricated sensor to detect chlorpyrifos. Under optimum conditions, the impedance value change of chlorpyrifos was proportional to its concentrations in the range of 100–105 ng/mL. The detection limit was found to be 0.014 ng/mL for chlorpyrifos. The proposed chlorpyrifos immunosensor could be used as a screening method in pesticide determination for the analysis of environmental, agricultural and pharmaceutical samples due to its rapidity, sensitivity and low cost.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30972055, 31101286), Agricultural Science and Technology Achievements Transformation Fund Projects of the Ministry of Science and Technology of China (No. 2011GB2C60020).

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Authors and Affiliations

  1. School of Agricultural and Food Engineering, Shandong University of Technology, No. 12, Zhangzhou Road, Zibo, 255049, Shandong, People’s Republic of China

    Yaoyao Cao, Xia Sun, Yemin Guo, Wenping Zhao & Xiangyou Wang

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  1. Yaoyao Cao
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  2. Xia Sun
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  3. Yemin Guo
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  4. Wenping Zhao
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  5. Xiangyou Wang
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Correspondence to Xia Sun.

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Cao, Y., Sun, X., Guo, Y. et al. An electrochemical immunosensor based on interdigitated array microelectrode for the detection of chlorpyrifos. Bioprocess Biosyst Eng 38, 307–313 (2015). https://doi.org/10.1007/s00449-014-1269-3

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  • DOI: https://doi.org/10.1007/s00449-014-1269-3

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