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Experimental analysis of the oil addition effect on mycelia and polysaccharide productions in Ganoderma lucidum submerged culture

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Abstract

The effect of corn oil addition on mycelium growth and polysaccharide productions in the medicinal mushroom Ganoderma lucidum was studied. The results showed that when a level of 2% corn oil was added at the beginning of culture, the biomass and polysaccharide productions reached a maximum of 12.9 and 1.038 g/L, respectively, during 13-day cultivation. The pH variation along with morphology observation in culture provided an indirect inference to the promotional effect of oil addition. Moreover, a curve fitting analysis was carried out to assay the elevated effect on biomass and exopolysaccharide productions in oil added culture. The experimental data of substrates consumption and products formation in culture with oil addition were predicted through the fitting equations obtained in single carbon source culture. The numerical results further clarified the stimulatory effects of oil addition in G. lucidum culture.

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Acknowledgments

This work was supported by a research-funding grant from National Science Council of Taiwan, R.O.C. (NSC-94-2214-E-005-003) and was also supported in part by the Ministry of Education, Taiwan, R.O.C. under the ATU plan.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Chung Hsing University, 250, Kuo Kuang Road, Taichung, 40227, Taiwan, ROC

    Hung-Chang Huang, Chih-I Chen, Chia-Nan Hung & Yung-Chuan Liu

  2. Department of Chemical Engineering and Biotechnology, Hsiuping Institute of Technology, No.11, Gongye Rd., Dali, Taichung County, 41280, Taiwan, ROC

    Chih-I Chen

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  1. Hung-Chang Huang
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Correspondence to Yung-Chuan Liu.

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Huang, HC., Chen, CI., Hung, CN. et al. Experimental analysis of the oil addition effect on mycelia and polysaccharide productions in Ganoderma lucidum submerged culture. Bioprocess Biosyst Eng 32, 217–224 (2009). https://doi.org/10.1007/s00449-008-0239-z

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  • DOI: https://doi.org/10.1007/s00449-008-0239-z

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