Skip to main content
SpringerLink
Log in

Molecular characterization of the Cryptosporidium cervine genotype from a sika deer (Cervus nippon Temminck) in Zhengzhou, China and literature review

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

A total of 124 fecal specimens were collected from four deer farms in Zhengzhou City, China and examined for Cryptosporidium by Sheather’s sugar flotation technique. Cryptosporidim oocysts were detected in two 1-year-old sika deer, and one of the two specimens was genotyped by sequence and phylogenetic analyses of the small subunit ribosomal RNA (rRNA) (18S rRNA), 70-kDa heat shock protein (HSP70), actin, and Cryptosporidium oocyst wall protein (COWP) genes. Results obtained suggested that the Cryptosporidium studied belonged to Cryptosporidium cervine genotype, although slight sequence differences were noticed at the three loci. The similarities between this isolate and other Cryptosporidium cervine genotype isolates were 99.1–99.8%, 9.8%, 99.7%, and 100% at the 18S rRNA, HSP70, actin, and COWP loci, respectively. This study is the first report of Cryptosporidium infection in sika deer in China.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Blackburn BG, Mazurek JM, Hlavsa M, Park J, Tillapaw M, Parrish M, Salehi E, Franks W, Koch E, Smith F, Xiao L, Arrowood M, Hill V, da Silva A, Johnston S, Jones JL (2006) Cryptosporidiosis associated with ozonated apple cider. Emerg Infect Dis 12:684–686

    PubMed  Google Scholar 

  • da Silva AJ, Cacciò S, Williams C, Won KY, Nace EK, Whittier C, Pieniazek NJ, Eberhard ML (2003) Molecular and morphologic characterization of a Cryptosporidium genotype identified in lemurs. Vet Parasitol 111:297–307

    Article  PubMed  Google Scholar 

  • Felsenstein J (1989) PHYLIP: phylogeny inference package (version 3.2). Cladistics 5:164–166

    Google Scholar 

  • Feltus DC, Giddings CW, Schneck BL, Monson T, Warshauer D, McEvoy JM (2006) Evidence supporting zoonotic transmission of Cryptosporidium spp. in Wisconsin. J Clin Microbiol 44:4303–4308

    Article  PubMed  CAS  Google Scholar 

  • Feng Y, Alderisio KA, Yang W, Blancero LA, Kuhne WG, Nadareski CA, Reid M, Xiao L (2007) Cryptosporidium genotypes in wildlife from a New York watershed. Appl Environ Microbiol 73:6475–6483

    Article  PubMed  CAS  Google Scholar 

  • Jiang J, Alderisio KA, Xiao L (2005) Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl Environ Microbio 171:4446–4454

    Article  CAS  Google Scholar 

  • Karanis P, Plutzer J, Halim NA, Igori K, Nagasawa H, Ongerth J, Liqing M (2007) Molecular characterization of Cryptosporidium from animal sources in Qinghai province of China. Parasitol Res 101:1575–1580

    Article  PubMed  Google Scholar 

  • Leoni F, Amar C, Nichols G, Pedraza-Diaz S, McLauchlin J (2006) Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000. J Med Microbiol 55:703–707

    Article  PubMed  CAS  Google Scholar 

  • Nichols GL, Chalmers RM, Sopwith W, Regan M, Hunter CA, Grenfell P, Harrison F, Lane C (2006) Cryptosporidiosis: a report on the surveillance and epidemiology of Cryptosporidium infection in England and Wales. Drinking Water Directorate Contract Number DWI 70/2/201. Drinking Water Inspectorate, UK, 142

  • Ong CS, Eisler DL, Alikhani A, Fung VW, Tomblin J, Bowie WR, Isaac-Renton JL (2002) Novel Cryptosporidium genotypes in sporadic cryptosporidiosis cases: first report of human infections with a cervine genotype. Emerg Infect Dis 8:263–268

    Article  PubMed  Google Scholar 

  • Perz JF, Le Blancq SM (2001) Cryptosporidium parvum infection involving novel genotypes in wildlife from lower New York State. Appl Environ Microbiol 67:1154–1162

    Article  PubMed  CAS  Google Scholar 

  • Ruecker NJ, Braithwaite SL, Topp E, Edge T, Lapen DR, Wilkes G, Robertson W, Medeiros D, Sensen CW, Neumann NF (2007) Tracking host sources of Cryptosporidium spp. in raw water for improved health risk assessment. Appl Environ Microbiol 73:3945–3957

    Article  PubMed  CAS  Google Scholar 

  • Ryan UM, Xiao L, Read C, Zhou L, Lal AA, Pavlasek I (2003) Identification of novel Cryptosporidium genotypes from the Czech Republic. Appl Environ Microbiol 69:4302–4307

    Article  PubMed  CAS  Google Scholar 

  • Ryan U, Bath C, Robertson I, Read C, Elliot A, McInnes L, Traub R, Besier B (2005) Sheep may not be an important zoonotic reservoir for Cryptosporidium and Giardia parasites. Appl Environ Microbiol 71:4992–4997

    Article  PubMed  CAS  Google Scholar 

  • Santin M, Trout JM, Fayer R (2007) Prevalence and molecular characterization of Cryptosporidium and Giardia species and genotypes in sheep in Maryland. Vet Parasitol 146:17–24

    Article  PubMed  CAS  Google Scholar 

  • Soba B, Petrovec M, Mioc V, Logar J (2006) Molecular characterisation of Cryptosporidium isolates from humans in Slovenia. Clin Microbiol Infect 12:918–921

    Article  PubMed  CAS  Google Scholar 

  • Trotz-Williams LA, Martin DS, Gatei W, Cama V, Peregrine AS, Martin SW, Nydam DV, Jamieson F, Xiao L (2006) Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario. Parasitol Res 99:346–352

    Article  PubMed  CAS  Google Scholar 

  • Xiao L, Escalante L, Yang C, Sulaiman I, Escalante AA, Montali RJ, Fayer R, Lal AA (1999) Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol 65:1578–1583

    PubMed  CAS  Google Scholar 

  • Xiao L, Aldeeisio K, Limor J, Royer M, Lal AA (2000) Identification of species and sources of Cryptopsporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl Environ Microbiol 66:5492–5498

    Article  PubMed  CAS  Google Scholar 

  • Xiao LH, Fayer R, Ryan U, Upton SJ (2004) Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol 17:72–97

    Article  Google Scholar 

  • Xiao L, Alderisio K, Singh A (2006a) Development and standardization of a Cryptosporidium genotyping tool for water samples. Awwa Research Foundation, Denver, CO

    Google Scholar 

  • Xiao L, Alderisio KA, Jiang J (2006b) Detection of cryptosporidium oocysts in water: effect of the number of samples and analytic replicates on test results. Appl Environ Microbiol 72:5942–5947

    Article  PubMed  CAS  Google Scholar 

  • Zhou L, Singh A, Jiang J, Xiao L (2003) Molecular surveillance of Cryptosporidium spp. in raw wastewater in Milwaukee: implications for understanding outbreak occurrence and transmission dynamics. J Clin Microbiol 41:5254–5257

    Article  PubMed  CAS  Google Scholar 

  • Ziegler PE, Wade SE, Schaaf SL, Chang YF, Mohammed HO (2007) Cryptosporidium spp. from small mammals in the New York city watershed. J Wildl Dis 43:586–596

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported in part by Henan Innovation Project for Prominent University Research Talents (number 2004KYCX002).

Author information

Authors and Affiliations

  1. The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People’s Republic of China

    Rongjun Wang, Jinchan Wang, Mingfei Sun, Hailiang Dang, Changshen Ning, Fuchun Jian & Longxian Zhang

  2. School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Yaoyu Feng

  3. US Department of Health and Human Services, Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA, 30341, USA

    Lihua Xiao

Authors
  1. Rongjun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jinchan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mingfei Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hailiang Dang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yaoyu Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Changshen Ning
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fuchun Jian
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Longxian Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Lihua Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Longxian Zhang or Lihua Xiao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, R., Wang, J., Sun, M. et al. Molecular characterization of the Cryptosporidium cervine genotype from a sika deer (Cervus nippon Temminck) in Zhengzhou, China and literature review. Parasitol Res 103, 865–869 (2008). https://doi.org/10.1007/s00436-008-1069-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-008-1069-2

Keywords

Search

Navigation