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The failure of Haemonchus contortus excretory/secretory products to stimulate gastrin secretion in vitro

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Abstract

Excretory/secretory (ES) products collected from exsheathed L3 or parasitic stages of Haemonchus contortus were tested in vitro for gastrin stimulatory properties using an ovine abomasal antral mucosal preparation. In addition, the motility of exsheathed L3 and parasites recovered on weeks 2, 6 and 8 post-infection was studied in water, saline, saline with glucose or ovine blood and in HBSS pH 2.5–7.4. Parasitic stages became immotile rapidly in water and HBSS pH 2.5, more slowly in HBSS pH 3.5, but nearly 100% remained motile for 48 h when blood was included in the medium. Exsheathed L3 motility was reduced only by water and HBSS pH 2.5, and then only in the second week of incubation. Gastrin secretion was not consistently increased by any of the parasite ES products tested in vitro, therefore, they probably do not stimulate the G cell directly to produce the hypergastrinaemia seen in parasitised sheep.

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Acknowledgments

We wish to thank Dr J. Hansky for his generous gift of gastrin Ab 74 and The C. Alma Baker Trust, E.&C. Thoms Bequest and Massey University Research Fund are thanked for their financial support for the experimental work and personal support for D.E.B. Lawton. These experiments comply with the current laws of New Zealand.

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Authors and Affiliations

  1. Massey University, Private Bag 11-222, Palmerston North, New Zealand

    David Lawton & Heather V. Simpson

  2. Kumeu Veterinary Services Ltd, 64 Access Road, Kumeu, New Zealand

    Elke Haag

Authors
  1. Elke Haag
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  2. David Lawton
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  3. Heather V. Simpson
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Correspondence to Heather V. Simpson.

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Haag, E., Lawton, D. & Simpson, H.V. The failure of Haemonchus contortus excretory/secretory products to stimulate gastrin secretion in vitro. Parasitol Res 95, 155–160 (2005). https://doi.org/10.1007/s00436-004-1274-6

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  • DOI: https://doi.org/10.1007/s00436-004-1274-6

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