Abstract
Ribosomes, ribosomal proteins (r-proteins), and messenger and transfer RNAs catalyze the synthesis of proteins in organisms. To understand and define the components involved in this event in Taenia solium, we isolated and characterized a T. solium cDNA encoding the basic ribosomal protein S15a (TsS15a). The TsS15a cDNA produces a protein with M r (relative molecular mass) 14,988, which contains 22.3% of basic amino acids. Analysis comparing TsS15a protein with other S15a r-proteins indicates that this protein is highly conserved. A recombinant TsS15a protein with similar M r was produced in bacteria. Antibodies against recombinant TsS15a react with a 15-kDa protein in extracts from all life stages of T. solium and from all helminths tested. Hybridization studies showed the presence of two genes encoding a mRNA of 0.5 kb. Moreover, the gene presents an intron of 30 bp. Our phylogenetic analysis using S15a r-proteins reproduced the topologies reported for 16/18S rRNA.
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Acknowledgements
This study was partially supported by grants IN218198 and IN213598 from the Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica of the Dirección General de Asuntos del Personal Académico, UNAM, and the Consejo Nacional de Ciencia y Tecnología grant 35333-M. The authors declare that the experiments performed in this work comply with the current ethical laws of the Universidad Nacional Autónoma de México.
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Nucleotide sequences reported in this paper have been deposited in the GenBank database under the accession numbers AF225905 (Taenia solium ribosomal protein S15a cDNA), AY196206 and AY196207 (genomic DNA fragments from T. solium and T. saginata, respectively).
An erratum to this article can be found at http://dx.doi.org/10.1007/s00436-004-1089-5
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Jiménez, L., Becerra, A. & Landa, A. Cloning, expression and partial characterization of a gene encoding the S15a ribosomal protein of Taenia solium . Parasitol Res 92, 414–420 (2004). https://doi.org/10.1007/s00436-003-1021-4
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DOI: https://doi.org/10.1007/s00436-003-1021-4