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Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds

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Abstract

Impact of exogenous calcium and ethylene glycol tetraacetic acid (EGTA) supplement on chickpea (Cicer arietinum L.) germinating seeds exposed to cadmium stress for 6 days was studied. Ca and EGTA late treatment (3 days) alleviated growth inhibition and decreased Cd accumulation as well as lipid peroxidation and protein carbonylation in both root and shoot cells. Exogenous effector application relieved Cd-induced cell death which was associated with a constant level of ATP, which was considered as an apoptotic-like process. Redox balance was examined through the study of the redox state of pyridine nucleotide couples NAD+/NADH and NADP+/NADPH as well as their related oxidative [NAD(P)H-oxidase] and dehydrogenase (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and malate dehydrogenase) enzyme activities. The present research illustrated an ameliorative effect of Ca and EGTA on growth of Cd-exposed chickpea seedlings that occurs through the protection of sensitive cell sites from Cd-induced oxidation, namely membrane lipids and proteins, rather than the improvement of recycling capabilities of the cellular reducing power.

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Acknowledgments

This work was financially supported by the Tunisian Ministry of Higher Education and Scientific Research (UR11ES32) and by Graduate School of Environmental and Life Science, Okayama University (Japan). The authors wish to thank Mr. Bechir Azib and Mr. Abbes Oucherine for technical assistance.

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Authors and Affiliations

  1. Laboratory of Plant Toxicology and Molecular Biology of Microorganisms, Faculty of Science of Bizerta, University of Carthage, 7021, Zarzouna, Tunisia

    Lamia Sakouhi, Sondès Rahoui, Marouane Ben Massoud, Ezzeddine EL Ferjani & Abdelilah Chaoui

  2. Graduate School of Environmental and Life Science, Okayama University, Okayama, 700-8530, Japan

    Shintaro Munemasa & Yoshiyuki Murata

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  1. Lamia Sakouhi
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  2. Sondès Rahoui
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  6. Yoshiyuki Murata
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  7. Abdelilah Chaoui
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Correspondence to Lamia Sakouhi.

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Sakouhi, L., Rahoui, S., Ben Massoud, M. et al. Calcium and EGTA Alleviate Cadmium Toxicity in Germinating Chickpea Seeds. J Plant Growth Regul 35, 1064–1073 (2016). https://doi.org/10.1007/s00344-016-9605-2

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  • DOI: https://doi.org/10.1007/s00344-016-9605-2

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