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Temperature preferences of bacteria isolated from seawater collected in Kandalaksha Bay, White Sea, Russia

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Abstract

Fifty-two bacteria were isolated from seawater collected in Kandalaksha Bay, White Sea, Russia, and classified by 16S rDNA sequencing. Most of the strains belonged to ubiquitous microorganisms. Pseudomonas was the most abundant genus (21 strains), including species of P. fluorescens, P. putida and P. syringae. Serratia was also common (10 strains) with species S. plymuthica and S. proteamaculans. Sphingobacterium, Flavobacterium and Pantoea were less represented (5, 3 and 2 strains, respectively). The only typical bacterium of marine Arctic regions was Shewanella baltica. The strains were tested for their optimal growth temperature in the range 0–45°C. The majority appeared to be psychrotolerant (42%) or mesophilic-psychrotolerant (40%). In addition, one strain (Bacillus pumilus) showed a rather narrow mesophilic profile. No true psychrophilic bacteria were found. Most of the strains showed a classical curve with fast growth decrease above the optimum; some others displayed uncommon flat curves with scarce differences between maximum and minimum of growth in a wide range of temperatures. Moreover, few strains presented an unusual profile being, in relation to the optimum, more tolerant to high rather than low temperatures. Preferences of the Kandalaksha Bay strains are generally different from those reported in literature for the same species: optima were at lower temperatures and, sometimes, ranges were broader showing increased eurythermism. This could indicate adaptation to the wide temperature variations recorded in this peculiar environment.

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Acknowledgments

The authors wish to thank Prof. A. Tzetlin and Dr. A. Zhadan, Department of Biology, Lomonosov Moscow State University, for the kind support during the sampling campaign carried out at the “White Sea Biological Station,” Kandalaksha Bay, Russia. The research was partially financed by the Italian-Russian Institute of Ecological Formation and Research (IIRFRE, Istituto Italo-Russo di Formazione e Ricerche Ecologiche).

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  1. DEB, Dipartimento di Scienze Ecologiche e Biologiche, University of Tuscia, Largo Università snc, 01100, Viterbo, Italy

    Chiara Pesciaroli, Francesco Cupini, Laura Selbmann, Paolo Barghini & Massimiliano Fenice

  2. Laboratory of Applied Marine Microbiology (Conisma), University of Tuscia, Largo Università snc, 01100, Viterbo, Italy

    Massimiliano Fenice

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  1. Chiara Pesciaroli
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  2. Francesco Cupini
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Correspondence to Massimiliano Fenice.

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Pesciaroli, C., Cupini, F., Selbmann, L. et al. Temperature preferences of bacteria isolated from seawater collected in Kandalaksha Bay, White Sea, Russia. Polar Biol 35, 435–445 (2012). https://doi.org/10.1007/s00300-011-1091-1

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