Abstract
CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28°C; but at 4°C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was transiently enhanced when cells were shifted from 22 to 4°C indicating that AAT is cold-inducible. Complementation of the mutated AAT transformed CSM2 from a cold-sensitive phenotype to a cold-resistant phenotype like the wild-type cells, thus providing evidence for the first time that AAT is required for low-temperature growth.
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Acknowledgments
Mr. Ashish would like to thank the Council of Scientific and Industrial Research, New Delhi, Government of India, for a Junior and Senior Research Fellowship. SS would like to thank CSIR for funding under the Network Project titled “Exploitation of India’s rich microbial diversity”. We are also thankful to Dr T N R Srinivas for help with construction of the phylogenetic trees.
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Communicated by Erko Stackebrandt.
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Sundareswaran, V.R., Singh, A.K., Dube, S. et al. Aspartate aminotransferase is involved in cold adaptation in psychrophilic Pseudomonas syringae . Arch Microbiol 192, 663–672 (2010). https://doi.org/10.1007/s00203-010-0591-7
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DOI: https://doi.org/10.1007/s00203-010-0591-7