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Archaeal DNA polymerases in biotechnology

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Abstract

DNA polymerase (pol) is a ubiquitous enzyme that synthesizes DNA strands in all living cells. In vitro, DNA pol is used for DNA manipulation, including cloning, PCR, site-directed mutagenesis, sequencing, and several other applications. Family B archaeal DNA pols have been widely used for molecular biological methods. Biochemical and structural studies reveal that each archaeal DNA pol has different characteristics with respect to fidelity, processivity and thermostability. Due to their high fidelity and strong thermostability, family B archaeal DNA pols have the extensive application on high-fidelity PCR, DNA sequencing, and site-directed mutagenesis while family Y archaeal DNA pols have the potential for error-prone PCR and random mutagenesis because of their low fidelity and strong thermostability. This information combined with mutational analysis has been used to construct novel DNA pols with altered properties that enhance their use as biotechnological reagents. In this review, we focus on the development and use of family B archaeal DNA pols.

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Acknowledgments

We thank Prof. A Steinbüchel for his support in this review. We also thank Prof. Linda J Reha-Krantz for the critical reading of our manuscript. Research in Dr. Likui Zhang’s laboratory was supported by the National Natural Science Foundation of China grant (No. 41306131), the Provincial Natural Science Foundation of Jiangsu, China grants (Nos. BK20130440 and 13KJB180029), the open project of State Key Laboratory of Microbial Resources (No. SKLMR-20130603), the Open-end Funds of Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology (No. 2014HS008), and the Yangzhou University College Student Science and Technology Innovation grant.

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, Yangzhou City, 225127, Jiangsu Province, China

    Likui Zhang, Manyu Kang, Jiajun Xu & Yanchao Huang

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  1. Likui Zhang
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  2. Manyu Kang
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  3. Jiajun Xu
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  4. Yanchao Huang
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Correspondence to Likui Zhang.

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Zhang, L., Kang, M., Xu, J. et al. Archaeal DNA polymerases in biotechnology. Appl Microbiol Biotechnol 99, 6585–6597 (2015). https://doi.org/10.1007/s00253-015-6781-0

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