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In vitro effect of temperature on the conformational structure and collagen binding of SdrF, a Staphylococcus epidermidis adhesin

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Abstract

Staphylococcus epidermidis is the leading etiologic agent of device-related infections. S. epidermidis is able to bind, by means of the adhesins of its cell wall, the host matrix proteins filming the artificial surfaces. Thence, bacteria cling to biomaterials and infection develops. The effect of temperature on integrity, structure, and biological activity of the collagen-binding adhesin (SdrF) of S. epidermidis has been here investigated. By cloning in E. coli XL1-Blue, a recombinant of the SdrF binding domain B (rSdrFB), carrying an N-terminal polyhistidine, was obtained. Purification was by HiTrapTM Chelating HP columns. Assessment of purity, molecular weight, and integrity was by SDS-PAGE. The rSdrFB-collagen binding was investigated by ELISA. A full three-dimensional reconstruction of rSdrFB was achieved by small-angle X-ray scattering (SAXS). At 25 °C, rSdrFB bound to type I collagen in a dose-dependent, saturable manner, with a Kd of 2.48 × 10−7 M. When temperature increased from 25 to 37 °C, a strong conformational change occurred, together with the abolition of the rSdrFB-collagen binding. The rSdrFB integrity was not affected by temperature variation. SdrFB-collagen binding is switched on/off depending on the temperature. Implications with the infection pathogenesis are enlightened.

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Acknowledgments

The authors are grateful for the assistance of the staff at ESRF Grenoble, France beamline BIO-SAXS ID14-3 (http://www.esrf.eu/news/spotlight/spotlight136/index_html) for data collection.

A financial contribution by “5 per mille” grants for Health Research to the Rizzoli Orthopedic Institute of Bologna is also acknowledged.

Conflict of interest

Columbia University has a patent for SdrF. Dr. Lowy is listed as one of the investigators on this patent.

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    Authors and Affiliations

    1. Department of Molecular Medicine, Center for Tissue Engineering (CIT), INSTM UdR of Pavia, University of Pavia, Viale Taramelli 3/b, 27100, Pavia, Italy

      Antonella Di Poto & Livia Visai

    2. Physics Institute, Sacred Heart Catholic University, Largo F. Vito, 1, 00168, Rome, Italy

      Massimiliano Papi, Alessandro Maiorana & Marco De Spirito

    3. Division of Infectious Diseases, Department of Medicine, College of Physicians and Surgeons, Columbia University, 630 West 168th Street Box 82, New York, NY, 10032, USA

      Sheetal Trivedi & Franklin D. Lowy

    4. Institute of Biophysics of the National Research Council of Italy, Via De Marini 6, 16149, Genoa, Italy

      Paola Gavazzo & Massimo Vassalli

    5. Research Unit on Implant Infections, Rizzoli Orthopedic Institute, via di Barbiano 1/10, 40136, Bologna, Italy

      Lucio Montanaro & Carla Renata Arciola

    6. Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, via San Giacomo 14, 40126, Bologna, Italy

      Lucio Montanaro & Carla Renata Arciola

    7. Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Via Forlanini 2, 27100, Pavia, Italy

      Marcello Imbriani

    8. Department of Occupational Medicine, Ergonomics and Disability, Laboratory of Nanotechnology, Salvatore Maugeri Foundation (FSM), Via Severino Boezio 28, 27100, Pavia, Italy

      Marcello Imbriani & Livia Visai

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    1. Antonella Di Poto
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    4. Alessandro Maiorana
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    Correspondence to Carla Renata Arciola or Livia Visai.

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    Antonella Di Poto and Massimiliano Papi contributed equally to this work.

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    Di Poto, A., Papi, M., Trivedi, S. et al. In vitro effect of temperature on the conformational structure and collagen binding of SdrF, a Staphylococcus epidermidis adhesin. Appl Microbiol Biotechnol 99, 5593–5603 (2015). https://doi.org/10.1007/s00253-015-6456-x

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