Abstract
In this work, the extracellular decolorization of aniline blue, a sulfonated triphenylmethane dye, by Shewanella oneidensis MR-1 was confirmed. S. oneidensis MR-1 showed a high capacity for decolorizing aniline blue even at a concentration of up to 1,000 mg/l under anaerobic conditions. Maximum decolorization efficiency appeared at pH 7.0 and 30 °C. Lactate was a better candidate of electron donor for the decolorization of aniline blue. The addition of nitrate, hydrous ferric oxide, or trimethylamine N-oxide all could cause a significant decline of decolorization efficiency. The Mtr respiratory pathway was found to be involved into the decolorization of aniline blue by S. oneidensis MR-1. The toxicity evaluation through phytotoxicity and genotoxicity showed that S. oneidensis MR-1 could decrease the toxicity of aniline blue during the decolorization process. Thus, this work may facilitate a better understanding on the degradation mechanisms of the triphenylmethane dyes by Shewanella and is beneficial to their application in bioremediation.
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Acknowledgments
The authors thank Dr. Emilia Botello (University of Extremadura) and Prof. K. H. Nealson (University of Southern California) for kindly providing the bacterial strains E. coli JC19008 and S. oneidensis MR-1, respectively. This study was partially supported by the National Natural Science Foundation of China (20907050 and 30970556) and the grant from Jiangsu University, China (10JDG126 and 09JDG020).
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Wu, Y., Xiao, X., Xu, C. et al. Decolorization and detoxification of a sulfonated triphenylmethane dye aniline blue by Shewanella oneidensis MR-1 under anaerobic conditions. Appl Microbiol Biotechnol 97, 7439–7446 (2013). https://doi.org/10.1007/s00253-012-4476-3
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DOI: https://doi.org/10.1007/s00253-012-4476-3