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Antihypertensive effects of Ile–Pro–Ile–Lys from krill (Euphausia superba) protein hydrolysates: purification, identification and in vivo evaluation in spontaneously hypertensive rats

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Abstract

Angiotensin I-converting enzyme (ACE) inhibitory peptide was purified from krill (Euphausia superba) protein hydrolysate using consecutive chromatography using an SP-Sephadex C-25 cation exchanger and then reverse-phase high-performance liquid chromatography. The purified ACE inhibitory peptide was identified to be Ile–Pro–Ile–Lys with a molecular weight of 470.3 Da by LC–MS/MS. The IC50 value of Ile–Pro–Ile–Lys was 57.4 µM. Lineweaver–Burk plots suggest that Ile–Pro–Ile–Lys acts as a non-competitive inhibitor against ACE. Oral administration of Ile–Pro–Ile–Lys significantly (p < 0.05) decreased the systolic blood pressure of spontaneously hypertensive rats. Collectively, an antihypertensive peptide purified from krill protein hydrolysates may be useful as a food ingredient for treatment of hypertension and related diseases.

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Acknowledgments

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2012R1A1A2040566).

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    Authors and Affiliations

    1. Department of Food Technology and Nutrition, Chonnam National University, Yeosu, 550-749, Republic of Korea

      Soo Yeon Park, Eui Jeong Han, Ju Hyung Um, Ginnae Ahn & Chang-Bum Ahn

    2. Department of Marine-Bio Convergence Science, Pukyong National University, Busan, 608-739, Republic of Korea

      Jae-Young Je

    3. Department of Marine Life Science, Jeju National University, Jeju, 690-756, Republic of Korea

      Nalae Kang & You-Jin Jeon

    4. Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, 550-749, Republic of Korea

      Ginnae Ahn

    5. Division of Food and Nutrition, Chonnam National University, Gwangju, 550-757, Republic of Korea

      Chang-Bum Ahn

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    1. Soo Yeon Park
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    Correspondence to Ginnae Ahn or Chang-Bum Ahn.

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    In this study, the animals were maintained in accordance with Guidelines for Care and Use of Laboratory Animals of Chonnam National University.

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    Soo Yeon Park and Jae-Young Je have contributed equally to this work.

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    Park, S.Y., Je, JY., Kang, N. et al. Antihypertensive effects of Ile–Pro–Ile–Lys from krill (Euphausia superba) protein hydrolysates: purification, identification and in vivo evaluation in spontaneously hypertensive rats. Eur Food Res Technol 243, 719–725 (2017). https://doi.org/10.1007/s00217-016-2785-y

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    • DOI: https://doi.org/10.1007/s00217-016-2785-y

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