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Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat

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A physical map of Secale cereale chromosome 6R was constructed using deletion mapping, and a new stripe rust resistance gene Yr83 was mapped to the deletion bin of FL 0.73–1.00 of 6RL.

Abstract

Rye (Secale cereale L., RR) possesses valuable genes for wheat improvement. In the current study, we report a resistance gene conferring stripe rust resistance effective from seedling to adult plant stages located on chromosome 6R. This chromosome was derived from triticale line T-701 and also carries highly effective resistance to the cereal cyst nematode species Heterodera avenae Woll. A wheat-rye 6R(6D) disomic substitution line exhibited high levels of seedling resistance to Australian pathotypes of the stripe rust (Puccinia striiformis f. sp. tritici; Pst) pathogen and showed an even greater resistance to the Chinese Pst pathotypes in the field. Ten chromosome 6R deletion lines and five wheat-rye 6R translocation lines were developed earlier in the attempt to transfer the nematode resistance gene to wheat and used herein to map the stripe rust resistance gene. These lines were subsequently characterized by sequential multicolor fluorescence in situ hybridization (mc-FISH), genomic in situ hybridization (GISH), mc-GISH, PCR-based landmark unique gene (PLUG), and chromosome 6R-specific length amplified fragment sequencing (SLAF-Seq) marker analyses to physically map the stripe rust resistance gene. The new stripe rust resistance locus was located in a chromosomal bin with fraction length (FL) 0.73–1.00 on 6RL and was named Yr83. A wheat-rye translocation line T6RL (#5) carrying the stripe rust resistance gene will be useful as a new germplasm in breeding for resistance.

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Acknowledgments

This work was financially supported by the Grains Research and Development Corporation (GRDC), Australia. We thank Ms Dawn Verlin, formerly of University of Adelaide, for assistance in the maintenance of some of the germplasm and Prof. Robert A. McIntosh, University of Sydney, for comments on the manuscript.

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Authors and Affiliations

  1. Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, 2570, Australia

    Jianbo Li, Chongmei Dong, Richard Trethowan, Sami Hoxha & Peng Zhang

  2. School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, SA, 5064, Australia

    Ian Dundas

  3. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China

    Jianbo Li, Guangrong Li & Zujun Yang

Authors
  1. Jianbo Li
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  2. Ian Dundas
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  3. Chongmei Dong
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  4. Guangrong Li
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  5. Richard Trethowan
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  6. Zujun Yang
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  7. Sami Hoxha
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  8. Peng Zhang
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Contributions

ID and PZ conceived and designed the study. ID developed the deletion and translocation lines. JL, PZ, and CD conducted the molecular marker analysis. JL and PZ performed the FISH and GISH experiments. PZ, JL, and SH performed the seedling-stage rust tests. GL and ZY scored the adult-stage responses in China. JL, PZ, RT, and CD analyzed the data. JL, PZ, and ID wrote the manuscript. All authors reviewed the manuscript.

Corresponding authors

Correspondence to Ian Dundas or Peng Zhang.

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The authors declare that they have no conflicts of interest.

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Communicated by Albrecht E. Melchinger.

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Li, J., Dundas, I., Dong, C. et al. Identification and characterization of a new stripe rust resistance gene Yr83 on rye chromosome 6R in wheat. Theor Appl Genet 133, 1095–1107 (2020). https://doi.org/10.1007/s00122-020-03534-y

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