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Fluorescent AFLP fingerprinting of Monilinia fructicola

Fluoreszenz-AFLP-Fingerprinting von Monilinia fructicola

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Abstract

The fluorescent amplified fragment length polymorphism method (AFLP) has been successfully applied to one of the brown rot fungi species — Monilinia fructicola, which causes severe losses in stone fruit production. This is the first report on the use of AFLP methodology for studying genetic variability among different M. fructicola isolates. A total of 1256 scorable, well defined AFLP fragments were obtained with 20 primer pairs, of which 462 were polymorphic. An unweighted pair group (UPGMA) dendrogram was constructed based on Jaccard’s similarity coefficient. A high similarity of the two Malus isolates from the USA was observed, while the other three Prunus isolates, from Spain, Japan and New Zealand, formed a second, more variable group. Comparison of AFLP fingerprinting data between M. fructicola and M. laxa revealed completely different banding patterns and demonstrated the suitability of the AFLP marker system for cross species differentiation of brown rot fungi.

Zusammenfassung

Die Amplifikationsfragment-Längenpolymorphismen-Methode (AFLP) wurde erfolgreich am Braunfäuleerreger Monilinia fructicola angewandt, der hohe Verluste im Steinobstbau verursacht. Dies ist der erste Bericht über die Verwendung der AFLP zur Charakterisierung der genetischen Variabilität unterschiedlicher M.-fructicola-Isolate. Insgesamt 1256 differenzierbare, definierte AFLP-Fragmente, von denen 462 polymorph waren, konnten mit 20 Primerpaaren gewonnen werden. Ein nicht gewichtetes Paargruppen-Dendrogramm wurde mit Hilfe des Jaccard-Ähnlichkeitskoeffizienten konstruiert. Eine hohe Ähnlichkeit der beiden Malus-Isolate aus den USA wurde beobachtet, während die drei Prunus-Isolate aus Spanien, Japan und Neuseeland eine zweite, variablere Gruppe bildeten. Ein Vergleich der AFLP-Fingerprints von M. fructicola und M. laxa ergab vollkommen unterschiedliche Banden und zeigte die Eignung des AFLP-Markersystems zur Artdiagnose von Braunfäuleerregern.

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Authors and Affiliations

  1. Biotechnology, Statistics and Plant Breeding, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    T. Gril (Chair of Genetics), B. Javornik (Chair of Genetics) & J. Jakse

  2. Agricultural Engineering, Crop Production, Grassland and Pasture Management, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    F. Celar (Chair of Phytomedicine)

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  1. T. Gril
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  2. F. Celar
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  3. B. Javornik
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  4. J. Jakse
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Correspondence to J. Jakse.

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Gril, T., Celar, F., Javornik, B. et al. Fluorescent AFLP fingerprinting of Monilinia fructicola. J Plant Dis Prot 117, 168–172 (2010). https://doi.org/10.1007/BF03356355

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  • DOI: https://doi.org/10.1007/BF03356355

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