Skip to main content
SpringerLink
Log in

Effect of fatty acids on growth of conjugated-linoleic-acids-producing bacteria in rumen

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Microorganisms with high activity of linoleic acid Δ12-cis,Δ11-trans-isomerase were isolated from the digestive tract of ruminants and characterized. The isolate with the highest isomerase activity was identified asPseudobutyrivibrio ruminis. The susceptibility of this strain to 3 fatty acids added to the grow medium was determined. A significant inhibition of bacterial growth (during a 3-d period) by linoleic acid (0.1 %) and oleic acid (5 ppm) was observed; no inhibition was found in the presence of stearic acid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Belury M.A., Nickel K.P., Bird C.E., Wu Y.M.: Inhibition of mouse skin tumor promotion by dietary conjugated linoleate.Nutr. Cancer26, 149–157 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Chin S.F., Liu W., Storkson J.M., Ha Y.L., Pariza M.W.: Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens.J.Food Compos.Anal.5, 185–197 (1992).

    Article  CAS  Google Scholar 

  • Griinari J.M., Cori B.A., Lacy S.H., Chouinard P.Y., Nurmela K.V.V.: Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Δ9-desaturase.J.Nutr.130, 2285–2291 (2000).

    PubMed  CAS  Google Scholar 

  • Hayek M.G., Han S.N., Wu D.Y., Watkins B.A., Meydani M., Dorsey J.L., Smith D.E., Meydani S.N.: Dietary conjugated linoleic acid influences the immune response of young and old C57BL/6NCrlBR mice.J.Nutr.129, 32–38 (1999).

    PubMed  CAS  Google Scholar 

  • Heipieper H.J., Meinhardt F., Segura A.: Thecis-trans isomerase of unsaturated fatty acids inPseudomonas andVibrio: biochemistry, molecular biology and physiological function of a unique stress adaptive mechanism.FEMS Microbiol.Lett.229, 1–7 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Houseknecht K.L., Van den Heuvel J.P., Moya-Camarena S.Y., Portocarero C.P., Peck L.W., Nickel K.P., Belury M.A.: Dietary conjugated linoleic acid normalized impaired glucose tolerance in the Zucker diabetic fatty fa/fa rat.Biochem.Biophys.Res.Commun.244, 678–682 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Hunter W.J., Baker F.C., Rosenfeld I.S., Keyser J.B., Tove S.B.: Biohydrogenation of unsaturated fatty acids. Hydrogenation by cell-free preparations ofButyrivibrio fibrisolvens.J.Biol.Chem.251, 2241–2247 (1976).

    PubMed  CAS  Google Scholar 

  • Jenkins T.C.: Lipid metabolism in the rumen.J. Diary Sci.76, 3851–3863 (1993).

    Article  CAS  Google Scholar 

  • Kepler C.R., Tove S.B.: Linoleate Δ12-cis,Δ11-trans-isomerase.Meth.Enzymol.14, 105–109 (1966).

    Article  Google Scholar 

  • Kepler C.R., Tove S.B.: Biohydrogenation of unsaturated fatty acids. III. Purification and properties of a linoleate Δ12-cis,Δ11-trans-isomerase fromButyrivibrio fibrisolvens.J.Biol.Chem.242, 5686–5692 (1967).

    PubMed  CAS  Google Scholar 

  • Kepler C.R., Hirons K.P., Mcneil J.J., Tove S.B.: Intermediates and products of the biohydrogenation of linoleic acid byButyrivibrio fibrisolvens.J.Biol.Chem.241, 1350–1354 (1966).

    PubMed  CAS  Google Scholar 

  • Kepler C.R., Tucker W.P., Tove S.B.: Biohydrogenation of unsaturated fatty acids. IV. Substrate specificity and inhibition of linoleate Δ12-cis,Δ11-trans-isomerase fromButyrivibrio fibrisolvens.J.Biol.Chem.245, 3612–3620 (1970).

    PubMed  CAS  Google Scholar 

  • Kim Y.J., Liu R.H., Bond D.R., Rusell J.B.: Effect of linoleic acid concentration on conjugated linoleic acid producing byButyrivibrio fibrisolvens A38.Appl.Environ.Microbiol.66, 5226–5230 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Kopečný J., Zorec M., Mrázek J., Kobayasho Y., Marinšek-Logar R.:Butyrivibrio hungatei sp.nov. andPseudobutyrivibrio xylanivorans sp.nov., butyrate-producing bacteria from the rumen.Internat.J.Syst.Evol.Microbiol.53, 201–209 (2003).

    Article  CAS  Google Scholar 

  • Kramer J.K., Parodi P.W., Jensen G.R., Mosobba M.M., Yurawecz M.P., Adlof R.O.: Rumenic acid: a proposed common name for the major conjugated linoleic acid isomer found in natural products.Lipids33, 835 (1998).

    Article  PubMed  CAS  Google Scholar 

  • Lee K.N., Kritchevsky D., Pariza M.W.: Conjugated linoleic acid and atherosclerosis in rabbits.Atherosclerosis108, 19–25 (1994).

    Article  PubMed  CAS  Google Scholar 

  • Mrázek J., Kopečný J.: Development of competitive PCR for detection ofButyrivibrio fibrisolvens in the rumen.Folia Microbiol.46, 63–65 (2001).

    Article  Google Scholar 

  • Parodi P.W.: Cows milk fat components as potential anticarcinogenic agents.J.Nutr.127, 1055–1060 (1997).

    PubMed  CAS  Google Scholar 

  • Steinhart C.: Conjugated linoleic acid — the good news about animal fat.J.Chem.Educ.73, A302-A303 (1996).

    Article  CAS  Google Scholar 

  • Zheng C.J., Yoo J.S., Lee T.G., Cho H.Y., Kim Y.H., Kim W.G.: Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids.FEBS Lett.579, 5157–5162 (2005).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    I. Koppová, F. Lukáš & J. Kopečný

Authors
  1. I. Koppová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Lukáš
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Kopečný
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. Koppová.

Additional information

The study was supported by theGrant Agency of the Czech Republic (project no. 525/04/0573) and theInstitutional Research Plan of theInstitute of Animal Physiology and Genetics. Academy of Sciences of the Czech Republic (no. AV 0Z 5045 0515).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koppová, I., Lukáš, F. & Kopečný, J. Effect of fatty acids on growth of conjugated-linoleic-acids-producing bacteria in rumen. Folia Microbiol 51, 291–293 (2006). https://doi.org/10.1007/BF02931816

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02931816

Keywords

Search

Navigation