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The different roles of serum and calcium in the control of proliferation of BALB/C 3T3 mouse cells

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An Erratum to this article was published on 01 May 1976

Summary

Proliferatively inactive BALB/c 3T3 mouse cells in dense cultures initiate a growth-division cycle upon exposure to fresh calf serum in a low-calcium (0.01 µM) medium. If these calcium-deprived cells are not supplied with calcium sometime during the first 10 hours after serum stimulation, they will rapidly return to a proliferatively inactive state without initiating DNA synthesis. The prereplicative development of such stimulated calcium-deprived cells appears to stop at an advanced stage, because addition of calcium as late as 10 hours after serum exposure rapidly initiates DNA synthesis, and enables the culture’s DNA-synthetic activity subsequently to reach its peak value at the same time as in control cultures.

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References

  1. Aaronson, S. A., and G. J. Todaro. 1968. Development of 3T3 like lines from BALB/c mouse embryo cultures: Transformation susceptibility to SV40. J. Cell. Physiol. 72: 141–148.

    Article  PubMed  CAS  Google Scholar 

  2. Todaro, G. J., G. K. Lazar, and H. Green. 1965. The initiation of cell division in a contact-inhibited mammalian cell line. J. Cell. Physiol. 66: 325–334.

    Article  PubMed  CAS  Google Scholar 

  3. Holley, R. W., and J. A. Kiernan. 1971. Studies of serum factors required by 3T3 and SV3T3 cells. In: G. E. W. Wolstenholme and J. Knight (Eds.),Ciba Foundation Symposium—Growth Control in Cell Cultures. Churchill, London, pp. 3–10.

    Google Scholar 

  4. Burger, M. M. 1970. Proteolytic enzymes initiating cell division and escape from contact inhibition of growth. Nature 227: 170–171.

    Article  PubMed  CAS  Google Scholar 

  5. Boynton, A. L., J. F. Whitfield, R. J. Isaacs, and H. J. Morton. 1974. Control of 3T3 cell proliferation by calcium. In Vitro 10: 12–17.

    Article  PubMed  CAS  Google Scholar 

  6. Balk, S. D., J. F. Whitfield, T. Youdale, and A. C. Braun. 1973. Roles of calcium, serum, plasma and folic acid in the control of proliferation of normal and Rous sarcoma virus-infected chicken fibroblasts. Proc. Nat. Acad. Sci. (Wash.) 70: 675–679.

    Article  CAS  Google Scholar 

  7. Swierenga, S. H. H., J. P. MacManus, and J. F. Whitfield. 1976. Regulation by calcium of the proliferation of heart cells from young adult rats. In Vitro 12: 31–36.

    PubMed  CAS  Google Scholar 

  8. Whitfield, J. F., J. P. MacManus, R. H. Rixon, and S. D. Balk. 1973. Calcium, cyclic AMP and the control of cell proliferation—a review. In Vitro 8: 257–278.

    Article  PubMed  CAS  Google Scholar 

  9. Whitney, R. B., and R. M. Sutherland. 1972. Requirement for calcium ions in lymphocyte transformation stimulated by phytohemagglutinin. J. Cell. Physiol. 80: 329–338.

    Article  PubMed  CAS  Google Scholar 

  10. Luckasen, J. R., J. G. White, and J. H. Kersey. 1974. Mitogenic properties of a calcium ionophore, A23187. Proc. Nat. Acad. Sci., U.S.A. 71: 5088–5090.

    Article  CAS  Google Scholar 

  11. Borle, A., and F. Briggs. 1968. Microdetermination of calcium in biological material by automatic fluorometric titration. Anal. Chem. 40: 336–344.

    Article  Google Scholar 

  12. Whitfield, J. F., J. P. MacManus, and D. J. Gillan. 1973. Calcium-dependent stimulation by a phorbol ester (PMA) of thymic lymphoblast DNA synthesis and proliferation. J. Cell. Physiol. 82: 151–156.

    Article  PubMed  CAS  Google Scholar 

  13. Whitfield, J. F., J. P. MacManus, T. Youdale, and D. J. Franks. 1971. The roles of calcium and cyclic AMP in the stimulatory action of parathyroid hormone on thymic lymphocyte proliferation. J. Cell. Physiol. 78: 355–368.

    Article  PubMed  CAS  Google Scholar 

  14. Seifert, W., and P. S. Rudland. 1974. Cyclic nucleotides and growth control in cultured mouse cells: Correlation of changes in intracellular 3′,5′ cGMP concentration with a specific phase of the cell cycle. Proc. Nat. Acad. Sci., U.S.A. 71: 4920–4924.

    Article  CAS  Google Scholar 

  15. Rudland, P. S., D. Gospodarowicz, and W. Seifert. 1974. Activation of guanyl cyclase and intracellular cyclic GMP by fibroblast growth factor. Nature 250: 741–744.

    Article  PubMed  CAS  Google Scholar 

  16. Whitfield, J. F., J. P. MacManus, R. H. Rixon, A. L. Boynton, T. Youdale, and S. Swierenga. 1976. The positive control of cell proliferation by the interplay of calcium ions and cyclic nucleotides—a review. In Vitro 12: 1–18.

    PubMed  CAS  Google Scholar 

  17. MacManus, J. P., B. M. Braceland, T. Youdale, and J. F. Whitfield. 1973. Adrenergic antagonist and a possible link between the increase in cyclic adenosine 3′,5′-monophosphate and DNA synthesis during liver regeneration. J. Cell. Physiol 82: 157–164.

    Article  PubMed  CAS  Google Scholar 

  18. Short, J., R. F. Brown, A. G. Husakova, J. R. Ibertson, R. Zemel, and I. Lieberman. 1971. Induction of deoxyribonucleic acid synthesis in the liver of the intact animal. J. Biol. Chem. 247: 1757–1766.

    Google Scholar 

  19. Rixon, R. H., and J. F. Whitfield. 1975. The control of liver regeneration by parathyroid hormone and calcium. J. Cell. Physiol. in press.

  20. Borle, A. 1974. Cyclic AMP stimulation of calcium efflux from kidney, liver and heart mitochondria. J. Membrane Biol. 1: 221–236.

    Article  Google Scholar 

  21. Lehninger, A. L. 1964.The Mitochondria. Molecular Basis of Structure and Function. W.H. Benjamin, New York and Amsterdam.

    Google Scholar 

  22. Lehninger, A. L., E. Carafoli, and C. S. Rossi. 1967. Energy-linked ion movements in mitochondrial systems. Adv. Enzymol. 29: 259–320.

    PubMed  CAS  Google Scholar 

  23. Rasmussen, H., D. B. Goodman, and A. Tenenhouse. 1972. The role of cyclic AMP and calcium in cell activation. C. R. C. Critical Rev. Biochem. 1: 95–148.

    Article  CAS  Google Scholar 

  24. Youdale, T., and J. P. MacManus. 1975. Failure of tritiated thymidine incorporation into DNA to reflect the autoradiographically demonstrable calcium-induced increase in thymic lymphoblast DNA synthesis. J. Cell. Physiol. in press.

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Authors and Affiliations

  1. Animal and Cell Physiology Group, Division of Biological Sciences, National Research Council of Canada, KIA 0R6, Ottawa, Canada

    A. L. Boynton, J. F. Whitfield & R. J. Isaacs

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  1. A. L. Boynton
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  2. J. F. Whitfield
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  3. R. J. Isaacs
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Additional information

Issued as NRCC No. 14999.

An erratum to this article is available at http://dx.doi.org/10.1007/BF02796319.

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Boynton, A.L., Whitfield, J.F. & Isaacs, R.J. The different roles of serum and calcium in the control of proliferation of BALB/C 3T3 mouse cells. In Vitro Cell.Dev.Biol.-Plant 12, 120–123 (1976). https://doi.org/10.1007/BF02796358

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