Skip to main content

Advertisement

SpringerLink
Log in

Semaphorin 3A Negatively Affects Proliferation of Mouse Thymus Epithelial Cells In Vitro

  • PHARMACOLOGY AND TOXICOLOGY
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied effects of semaphorin 3A, keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), and their combinations on the proliferative activity of cortical (cTEC1-2) and medullary (mTEC3-10) thymus epithelium cell lines. Semaphorin 3A inhibited the proliferative activity of epithelial cells, while HGF and KGF, in contrast, exerted a stimulating effect. The effect of KGF and semaphorin 3A on different cell lines depended on the expression of receptors for these two factors. When the combination of two factors was used, semaphorin 3A was able to neutralize the stimulating effect of HGF and KGF. It can be assumed that semaphorin 3A synthesized in the thymus stroma, can act as a functional antagonist of HGF and KGF and have an inhibitory effect when these drugs are administered into the body for the therapeutic purpose of restoring thymus functions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kisseleva EP, Lyamina IV, Tzvirkun SA, Rutto KV, Kudryavtsev IV, Lioudyno VI. Investigation of the role of semaphorin 3a in the thymus at normal conditions and tumor growth. Med. Akad. Zh. 2013;13(4):42-48. Russian.

  2. Abramson J, Anderson G. Thymic epithelial cells. Annu. Rev. Immunol. 2017;35:85-118.

    Article  CAS  PubMed  Google Scholar 

  3. Catalano A, Caprari P, Moretti S, Faronato M, Tamagnone L, Procopio A. Semaphorin-3A is expressed by tumor cells and alters T-cell signal transduction. Blood. 2006;107(8):3321-3329.

    Article  CAS  PubMed  Google Scholar 

  4. Dent MF, Hubbold L, Radford H, Wilson AP. The methylene blue colorimetric microassay for determining cell line response to growth factors. Cytotechnology. 1995;17(1):27-33.

    Article  CAS  PubMed  Google Scholar 

  5. Erickson M, Morkowski S, Lehar S, Gillard G, Beers C, Dooley J, Rubin JS, Rudensky A, Farr AG. Regulation of thymic epithelium by keratinocyte growth factor. Blood. 2002;100(9):3269-3278.

    Article  CAS  PubMed  Google Scholar 

  6. Gray DH, Tull D, Ueno T, Seach N, Classon BJ, Chidgey A, McConville MJ, Boyd RL. A unique thymic fibroblast population revealed by the monoclonal antibody MTS-15. J. Immunol. 2007;178(8):4956-4965.

    Article  CAS  PubMed  Google Scholar 

  7. Imado T, Iwasaki T, Kataoka Y, Kuroiwa T, Hara H, Fujimoto J, Sano H. Hepatocyte growth factor preserves graft-versus-leukemia effect and T-cell reconstitution after marrow transplantation. Blood. 2004;104(5):1542-1549.

    Article  CAS  PubMed  Google Scholar 

  8. Jung WS, Han SM, Kim SM, Kim ME, Lee JS, Seo KW, Youn HY, Lee HW. Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model. Cell Biol. Int. 2014;38(10):1106-1117.

    Article  CAS  PubMed  Google Scholar 

  9. Kasai M, Mizuochi T. Culture and characterization of thymic epithelial cell lines. Methods Mol. Biol. 2007;380:107-123.

    Article  CAS  PubMed  Google Scholar 

  10. Lepelletier Y, Moura I.C, Hadj-Slimane R, Renand A, Fiorentino S, Baude C, Shirvan A, Barzilai A, Hermine O. Immunosuppressive role of semaphorin-3A on T cell proliferation is mediated by inhibition of actin cytoskeleton reorganization. Eur. J. Immunol. 2006;36(7):1782-1793.

    Article  CAS  PubMed  Google Scholar 

  11. Lepelletier Y, Smaniotto S, Hadj-Slimane R, Villa-Verde DM, Nogueira AC, Dardenne M, Hermine O, Savino W. Control of human thymocyte migration by Neuropilin-1/Semaphorin-3A-mediated interactions. Proc. Natl Acad. Sci. USA. 2007;104(13):5545-5550.

    Article  CAS  PubMed  Google Scholar 

  12. Rossi SW, Jeker LT, Ueno T, Kuse S, Keller MP, Zuklys S, Gudkov AV, Takahama Y, Krenger W, Blazar BR, Holländer GA. Keratinocyte growth factor (KGF) enhances postnatal T-cell development via enhancements in proliferation and function of thymic epithelial cells. Blood. 2007;109(9):3803-3811.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Skibinski G, Skibinska A, James K. The role of hepatocyte growth factor and its receptor c-met in interactions between lymphocytes and stromal cells in secondary human lymphoid organs. Immunology. 2001;102(4):506-514.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Takamatsu H, Kumanogoh A. Diverse roles for semaphorin-plexin signaling in the immune system. Trends Immunol. 2012;33(3):127-135.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Experimental Medicine, St. Petersburg, Russia

    K. V. Rutto, K. S. Ovsyukov, I. V. Kudryavtsev & E. P. Kiseleva

  2. I. I. Mechnikov North-Western State Medical University, St. Petersburg, Russia

    E. P. Kiseleva

Authors
  1. K. V. Rutto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. S. Ovsyukov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. V. Kudryavtsev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. P. Kiseleva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. V. Rutto.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 9, pp. 310-314, September, 2018

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rutto, K.V., Ovsyukov, K.S., Kudryavtsev, I.V. et al. Semaphorin 3A Negatively Affects Proliferation of Mouse Thymus Epithelial Cells In Vitro. Bull Exp Biol Med 166, 339–343 (2019). https://doi.org/10.1007/s10517-019-04346-x

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-019-04346-x

Key Words

Search

Navigation