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Ancestral remnants in deoxyribonucleic acid fromPseudomonas andXanthomonas

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Abstract

Reciprocal hybridizations were carried out between DNA fromPseudomonas fluorescens, P. putida and a xanthomonad,P. campestris var.pelargonii. Furthermore, DNA-fragments from each organism, preselected by hybridization with each of the other two strains, were again hybridized with all three DNA-types. The molecular weight of the chromosomal DNA from the three organisms is about equal, having a value of 2.4·0.4×109 daltons/nucleoid or 3.9 × 106 nucleotide pairs/nucleoid. About half of the DNA from each organism has a similar, but not identical, nucleotide sequence. Theputida- andfluorescens-DNA share an additional 33°. homology. From the present and previous experiments it can be hypothesized that all xanthomonads share an additional stretch of homologous DNA, amounting to some 25–40% of the bacterial chromosome. The remaining DNA in each organism (about 15%) is probably responsible for strain individuality (varieties, races and strains within the same genospecies). The results suggest that the three organisms are derived from a common pool of ancestors; that the xanthomonads diverged first or fastest and that the split betweenP. putida andP. fluorescens is a more recent event. The mean molar (guanine + cytosine) content of the common part is identical to that of the total parent DNA. The average value for the three organisms is 62·2% (G+C). The direction of the evolutionary drift of the (G+C) content between these closely related organisms is not detectable.

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Authors and Affiliations

  1. Laboratory for Microbiology, Faculty of Sciences, State University, Gent, Belgium

    I. W. Park & J. De Ley

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  1. I. W. Park
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  2. J. De Ley
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Park, I.W., De Ley, J. Ancestral remnants in deoxyribonucleic acid fromPseudomonas andXanthomonas . Antonie van Leeuwenhoek 33, 1–16 (1967). https://doi.org/10.1007/BF02045528

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  • DOI: https://doi.org/10.1007/BF02045528

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