Abstract
Purine biosynthesis was studied in 8 mutants of Chinese hamster cells which require purines for growth and in wild-type cells which do not show this nutritional requirement. Of these, 6 mutants, ade −B, ade−D, ade−E, ade−F, GAT−, and AT−, were shown to accumulate metabolic intermediates not accumulated by wild-type cells. These intermediates were shown to be compounds unique to the adenylic acid biosynthetic pathway by the following criteria: (a) their radioisotopic labeling properties, (b) their response to agents which specifically inhibit known enzymatic steps in the pathway, (c) their chromatographic properties, and (d) spectrophotometric analysis. Two mutants, ade−A and ade−C, accumulate no detectable compounds not accumulated by the wild type. These 2 mutants are believed to be defective in steps very early in the purine biosynthetic pathway. The sites of the defects in the other mutants are proposed, and the usefulness of these mutants is discussed.
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Literature cited
Kao, F. T., and Puck, T. T. (1968).Proc. Natl. Acad. Sci. 60:1275–1281.
Hartman, S. C., and Buchanan, J. M. (1959).Ann. Rev. Biochem. 28:365–410.
Magasanik, B. (1963). In Gunsalus, J. C., and Stanier, R. Y. (eds.),The Bacteria, Vol. 3, Academic Press, New York, pp. 295–334.
Westby, C. A., and Gots, J. S. (1969).J. Biol. Chem. 244:2095–2102.
Lukens, L., and Flaks, J. G. (1963). In Colowick, S. P., and Kaplan, N. O. (eds.),Methods in Enzymology, Vol. 6, Academic Press, New York, pp. 671–702.
Flaks, J. G., and Lukens, L. (1963). In Colowick, S. P., and Kaplan, N. O., (eds.),Methods in Enzymology, Vol. 6, Academic Press, New York, pp. 52–99.
Henderson, J. F. (1956).J. Biol. Chem. 240(7):3104–3109.
Green, C. D., and Martin, D. W. (1973).Proc. Natl. Acad. Sci. 70:3698–3702.
Henderson, J. F., and Khoo, M. K. Y. (1965).J. Biol. Chem. 240(6):2358–2362.
Martin, D. W., and Owen, N. T. (1972).J. Biol. Chem. 247(17):5477–5485.
Reem, G. H., and Friend, C. (1967).Science 157:1203–1204.
Becker, M. A., Kostel, P. J., and Meyer, L. J. (1973).Proc. Natl. Acad. Sci. 70:2749–2752.
Wood, H. A., Becker, M. A., and Seegmiller, J. E. (1973).Biochem. Genet. 9:261–274.
Rosenbloom, F. M., Henderson, J. F., Caldwell, I. C., Kelley, W. N., and Seegmiller, J. E. (1968).J. Biol. Chem. 243(6): 1166–1173.
Lesch, M., and Nyhan, W. T. (1964).Am. J. Med. 36:561–570.
Nyhan, W. T., James, J. A., Teberg, A. J., Sweetman, L., and Nelson, L. G. (1969).J. Pediat. 74(1):20–27.
Patterson, D., Kao, F. T., and Puck, T. T. (1974).Proc. Natl. Acad. Sci. 71:2057–2061.
Ham, R. G. (1965).Proc. Natl. Acad. Sci. 53:288–293.
Ham, R. G., and Puck, T. T. (1962). In Colowick, S. P., and Kaplan, N. O. (eds.),Methods in Enzymology, Vol. 5, Academic Press, New York, pp. 90–119.
Boyle, J. A., Raivio, K. O., Becker, M. A., and Seegmiller, J. E. (1972).Biochim. Biophys. Acta 269:179–183.
Bratton, A. C., and Marshall, E. K. (1939).J. Biol. Chem. 128:537–550.
Shantz, G. D., Fontenelle, L. J., and Henderson, J. F. (1972).Biochem. Phamacol. 21(8): 1203–1206.
Hill, D. L., and Bennett, L. L. (1969).Biochemistry 8:122–130.
Flaks, J. G., Erwin, M. J., and Buchanan, J. M. (1957).J. Biol. Chem. 228:201–213.
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Patterson, D. Biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism: Biochemical analysis of eight mutants. Somat Cell Mol Genet 1, 91–110 (1975). https://doi.org/10.1007/BF01538734
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DOI: https://doi.org/10.1007/BF01538734