Summary
The subcellular location of dehydrins was determined by immunomicroscopy in maize kernels imbibed in the presence of ABA. Antibodies were specific to the consensus sequence that exists near the carboxy terminus of virtually all dehydrins. Aleurone, scutellar epithelium, scutellar provascular strands, and the outermost embryonic leaves contained the highest concentration of dehydrins. In these tissues, and in scutellar parenchyma cells and inner embryonic leaves, dehydrins were present in the cytoplasm and associated with nuclei. In shoot and root apex cells, dehydrins appeared to be present principally in the cytoplasm. This localization pattern of dehydrins, together with the known amino acid sequences and phosphorylation patterns, leads to analogies with other proteins that are present in both the nucleus and cytoplasm of eukaryotic cells.
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Abbreviations
- ABA:
-
abscisic acid
- ER:
-
endoplasmic reticulum
- PMSF:
-
phenylmethylsulfonylfluoride
- PBS:
-
phosphate-buffered saline
- RT:
-
room temperature
- SDS-PAGE:
-
sodium dodecyl sulfate, polyacrylamide gel electrophoresis
- TES:
-
N-Tris(hydroxymethyl)methyl-2-aminoethane sulfonic acid
- Tris:
-
Tris(hydroxymethyl)aminomethane
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Asghar, R., Fenton, R.D., DeMason, D.A. et al. Nuclear and cytoplasmic localization of maize embryo and aleurone dehydrin. Protoplasma 177, 87–94 (1994). https://doi.org/10.1007/BF01378983
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DOI: https://doi.org/10.1007/BF01378983